PUBLICATION

Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics

Authors
Prajsnar, T.K., McVicker, G., Williams, A., Renshaw, S.A., Foster, S.J.
ID
ZDB-PUB-180113-7
Date
2018
Source
Methods in molecular biology (Clifton, N.J.)   1736: 147-156 (Chapter)
Registered Authors
Prajsnar, Thomasz, Renshaw, Steve A., Williams, Alex
Keywords
Bacterial population dynamics, Fluorescence microscopy, Infection, Zebrafish
MeSH Terms
  • Animals
  • Bacterial Infections/microbiology*
  • Bacterial Load
  • Disease Models, Animal
  • Embryo, Nonmammalian
  • Host-Pathogen Interactions*
  • Larva
  • Microscopy, Fluorescence
  • Zebrafish/microbiology*
PubMed
29322467 Full text @ Meth. Mol. Biol.
Abstract
Investigating bacterial dynamics within the infected host has proved very useful for understanding mechanisms of pathogenesis. Here we present the protocols we use to study bacterial dynamics within infected embryonic zebrafish. This chapter encompasses basic techniques used to study bacterial infection within larval zebrafish, including embryonic zebrafish maintenance, injections of morpholino oligonucleotides, intravenous injections of bacterial suspensions, and fluorescence imaging of infected zebrafish. Specific methods for studying bacterial within-host population dynamics are also described.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping