PUBLICATION

Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae

Authors
Su, B.C., Lai, Y.W., Chen, J.Y., Pan, C.Y.
ID
ZDB-PUB-180111-5
Date
2018
Source
Fish & shellfish immunology   74: 235-241 (Journal)
Registered Authors
Pan, Chieh-Yu
Keywords
Streptococcus agalactiae, Tilapia piscidin 3, Zebrafish
MeSH Terms
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/immunology
  • Animals, Genetically Modified/metabolism
  • Fish Proteins*/genetics
  • Fish Proteins*/metabolism
  • Animals
  • Disease Resistance/genetics*
  • Disease Resistance/immunology
  • Streptococcus agalactiae/physiology
  • Cichlids*/genetics
  • Cichlids*/immunology
  • Fish Diseases/genetics*
  • Fish Diseases/immunology*
  • Streptococcal Infections/genetics
  • Streptococcal Infections/immunology
  • Streptococcal Infections/veterinary*
  • Zebrafish/genetics*
  • Zebrafish/immunology
  • Zebrafish/metabolism
(all 19)
PubMed
29317307 Full text @ Fish Shellfish Immunol.
Abstract
To study the biological role of tilapia piscidin 3 (TP3) in Streptococcus agalactiae infection in vivo, TP3/DsRed overexpressing transgenic zebrafish were generated. Under normal growth conditions, TP3/DsRed transgenic zebrafish exhibited an orange-red body color, without any other obvious abnormalities. However, when compared to wild type fish, TP3/DsRed transgenic zebrafish were resistant to S. agalactiae infection. After infection, the TP3 overexpressing fish exhibited higher expression of Toll-like receptor 4a (TLR4a), interleukin (IL)-10, IL-22, and C3b. Furthermore, TP3/DsRed transgenic zebrafish exhibited reduced induction of proinflammatory cytokines, including TNF?, IL-1?, IL-21, MyD88, and nuclear factor (NF)-?B. Taken together, our data show that TP3 overexpression in zebrafish can effectively suppress proinflammatory responses and enhance production of C3b. Together, these actions are conducive to the resolution of inflammation and bacterial clearance. We further postulate that TP3 may exert its anti-inflammatory effects by enhancing TLR4a-mediated negative regulation of NF-?B.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Su, B.C., Lai, Y.W., Chen, J.Y., Pan, C.Y. (2018) Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae. Fish & shellfish immunology. 74:235-241.