PUBLICATION

Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish

Authors
Maheras, A.L., Dix, B., Carmo, O.M.S., Young, A.E., Gill, V.N., Sun, J.L., Booker, A.R., Thomason, H.A., Ibrahim, A.E., Stanislaw, L., Dallego, J.C., Ngo, C.N., Chen, A., Fortini, B.K., Spence, R.D.
ID
ZDB-PUB-180106-4
Date
2018
Source
eNeuro   5(1): (Journal)
Registered Authors
Keywords
Concussion, DEG, RNA-seq, TBI, mTBI, zebrafish
MeSH Terms
  • Male
  • Spatial Memory
  • Regeneration*
  • Disease Models, Animal
  • Zebrafish
  • Female
  • Gene Expression
  • Fish Proteins/genetics
  • Animals
  • Brain Concussion/genetics*
  • Brain/physiology*
  • Gene Ontology
(all 12)
PubMed
29302617 Full text @ eNeuro
Abstract
Mild traumatic brain injuries (mTBIs) are one of the most prevalent neurological disorders, and humans are severely limited in their ability to repair and regenerate central nervous system (CNS) tissue postinjury. However, zebrafish (Danio rerio) maintain the remarkable ability to undergo complete and functional neuroregeneration as an adult. We wish to extend knowledge of the known mechanisms of neuroregeneration by analyzing the differentially expressed genes (DEGs) in a novel adult zebrafish model of mTBI. In this study, a rodent weight drop model of mTBI was adapted to the adult zebrafish. A memory test showed significant deficits in spatial memory in the mTBI group. We identified DEGs at 3 and 21 days postinjury (dpi) through RNA-sequencing analysis. The resulting DEGs were categorized according to gene ontology (GO) categories. At 3 dpi, GO categories consisted of peak injury response pathways. Significantly, at 21 dpi, GO categories consisted of neuroregeneration pathways. Ultimately, these results validate a novel zebrafish model of mTBI and elucidate significant DEGs of interest in CNS injury and neuroregeneration.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
dt2
    		Unknown
    1 - 1 of 1
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    brain compressionTAS
    1 - 1 of 1
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    Sequence Targeting Reagents
    No data available
    Fish
    No data available
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Maheras, A.L., Dix, B., Carmo, O.M.S., Young, A.E., Gill, V.N., Sun, J.L., Booker, A.R., Thomason, H.A., Ibrahim, A.E., Stanislaw, L., Dallego, J.C., Ngo, C.N., Chen, A., Fortini, B.K., Spence, R.D. (2018) Genetic Pathways of Neuroregeneration in a Novel Mild Traumatic Brain Injury Model in Adult Zebrafish. eNeuro. 5(1).