PUBLICATION
Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish
- Authors
- Arévalo, J.C., Hernández-Jiménez, E., Jiménez-González, A., Torres-Valle, M., Iwasaki, R.S., López-Bellido, R., Vicente-García, C., Rodríguez, R.E.
- ID
- ZDB-PUB-180106-1
- Date
- 2018
- Source
- International Journal of Molecular Sciences 19(1): (Journal)
- Registered Authors
- Keywords
- antibodies, opioid receptors, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Antibodies/metabolism*
- Antibody Specificity
- Female
- HEK293 Cells
- Humans
- Larva/metabolism
- Rabbits
- Receptors, Opioid/chemistry
- Receptors, Opioid/immunology*
- Receptors, Opioid, delta/metabolism
- Sequence Alignment
- Zebrafish/metabolism*
- PubMed
- 29301275 Full text @ Int. J. Mol. Sci.
Citation
Arévalo, J.C., Hernández-Jiménez, E., Jiménez-González, A., Torres-Valle, M., Iwasaki, R.S., López-Bellido, R., Vicente-García, C., Rodríguez, R.E. (2018) Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish. International Journal of Molecular Sciences. 19(1).
Abstract
The opioid system is well conserved among species and plays a critical role in pain and addiction systems. The use of zebrafish as an experimental model to study development and genetics is extraordinary and has been proven to be relevant for the study of different diseases. The main drawback to its use for the analysis of different pathologies is the lack of protein tools. Antibodies that work in other models are not suitable for zebrafish due to the low degree of homology that exists among the opioid receptor protein sequences in different species. Here we report the successful generation and characterization of antibodies against the mu, delta 1 and delta 2 opioid receptors in zebrafish. The antibodies obtained, which are specific for each receptor due to the use of the C-terminus as antigens, work for Western blotting and immunohistochemistry. In addition, the antibodies against mu and delta 1 opioid receptors, but not those against delta 2, are able to immunoprecipitate the corresponding receptor from zebrafish lysates. The development of opioid receptor antibodies is an asset to the further study of the endogenous opioid system in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping