ZFIN ID: ZDB-PUB-171230-5
Cell-accurate optical mapping across the entire developing heart
Weber, M., Scherf, N., Meyer, A.M., Panáková, D., Kohl, P., Huisken, J.
Date: 2017
Source: eLIFE   6: (Journal)
Registered Authors: Huisken, Jan, Meyer, Alexander, Panáková, Daniela
Keywords: Cardiac conduction, Cardiology, Light sheet microscopy, Optical mapping, Organogenesis, biophysics, developmental biology, image analysis, stem cells, structural biology, zebrafish
MeSH Terms:
  • Animals
  • Heart/embryology*
  • Imaging, Three-Dimensional/methods*
  • Intravital Microscopy/methods*
  • Optical Imaging/methods*
  • Organogenesis
  • Zebrafish/embryology*
PubMed: 29286002 Full text @ Elife
Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.