|ZFIN ID: ZDB-PUB-171230-11|
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
Ellett, F., Irimia, D.
|Source:||Journal of visualized experiments : JoVE (130): (Journal)|
|Registered Authors:||Ellett, Felix|
|PubMed:||29286475 Full text @ J. Vis. Exp.|
Ellett, F., Irimia, D. (2017) Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae. Journal of visualized experiments : JoVE. (130).
ABSTRACTZebrafish have emerged as a powerful model of various human diseases and a useful tool for an increasing range of experimental studies, spanning fundamental developmental biology through to large-scale genetic and chemical screens. However, many experiments, especially those related to infection and xenograft models, rely on microinjection and imaging of embryos and larvae, which are laborious techniques that require skill and expertise. To improve the precision and throughput of current microinjection techniques, we developed a series of microstructured devices to orient and stabilize zebrafish embryos at 2 days post fertilization (dpf) in ventral, dorsal, or lateral orientation prior to the procedure. To aid in the imaging of embryos, we also designed a simple device with channels that orient 4 zebrafish laterally in parallel against a glass cover slip. Together, the tools that we present here demonstrate the effectiveness of photolithographic approaches to generate useful devices for the optimization of zebrafish techniques.