PUBLICATION

Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry

Authors
Ery?lmaz, O., Ate?, P.S., Ünal, ?., Üstünda?, Ü.V., Bay, S., Alturfan, A.A., Yi?itba??, T., Emekli-Alturfan, E., Akal?n, M.
ID
ZDB-PUB-171229-3
Date
2017
Source
Journal of biochemical and molecular toxicology   32(1): (Journal)
Registered Authors
Ate?, Perihan Seda, Emekli-Alturfan, Ebru, Unal, Ismail, Üstünda?, Ünsal Veli
Keywords
Wnt pathway, apoptosis, oxidant-antioxidant status, silver nanoparticles, zebrafish embryo
MeSH Terms
  • Lipid Peroxidation/drug effects
  • Enzyme Induction/drug effects
  • Apoptosis/drug effects*
  • Gene Expression Regulation, Developmental/drug effects
  • Water Pollutants, Chemical/administration & dosage
  • Water Pollutants, Chemical/toxicity*
  • Embryonic Development/drug effects
  • Embryo, Nonmammalian/abnormalities
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism
  • Teratogens/toxicity
  • Cyclin D1/antagonists & inhibitors
  • Cyclin D1/genetics
  • Cyclin D1/metabolism
  • Animals
  • Cell Proliferation/drug effects
  • Oxidative Stress/drug effects*
  • Zebrafish
  • Metal Nanoparticles/administration & dosage
  • Metal Nanoparticles/toxicity*
  • Glycogen Synthase Kinase 3 beta/chemistry
  • Glycogen Synthase Kinase 3 beta/genetics
  • Glycogen Synthase Kinase 3 beta/metabolism
  • Zebrafish Proteins/agonists
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Dose-Response Relationship, Drug
  • Silver/administration & dosage
  • Silver/toxicity*
  • Wnt Signaling Pathway/drug effects*
  • Disinfectants/toxicity
(all 32)
PubMed
29283201 Full text @ J. Biochem. Mol. Toxicol.
CTD
29283201
Abstract
Antimicrobial textile products are developing rapidly as an important part of functional textiles. Silver nanoparticles (AgNPs) are nanotechnology products with antimicrobial properties. However, exposure to nanoparticles in daily life is an important issue for public health, still being updated. Aim was to evaluate the effects of AgNPs on the development of zebrafish embryos focusing on Wnt pathway, proliferation, oxidant-antioxidant status, and apoptosis. The expressions of ccnd1 and gsk3? were determined by RT-PCR, whereas ?-catenin and proliferative cell antigen (PCNA) expressions were determined immunohistochemically. Lipid peroxidation, superoxide dismutase, and glutathione-S-transferase activities were determined spectrophotometrically. Apoptosis was determined using acridine orange staining. Oxidant status, apoptosis, immunohistochemical PCNA, and ? catenin staining increased, whereas ccnd1 and antioxidant enzyme activities decreased in AgNPs-exposed embryos in a dose-dependent manner. Our results indicate the interaction of possible mechanisms that may be responsible for the toxic effects of AgNPs in zebrafish embryos.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Ery?lmaz, O., Ate?, P.S., Ünal, ?., Üstünda?, Ü.V., Bay, S., Alturfan, A.A., Yi?itba??, T., Emekli-Alturfan, E., Akal?n, M. (2017) Evaluation of the interaction between proliferation, oxidant-antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry. Journal of biochemical and molecular toxicology. 32(1).