PUBLICATION
A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish
- Authors
- Chou, S.W., Chen, Z., Zhu, S., Davis, R.W., Hu, J., Liu, L., Fernando, C.A., Kindig, K., Brown, W.C., Stepanyan, R., McDermott, B.M.
- ID
- ZDB-PUB-171223-3
- Date
- 2017
- Source
- Nature communications 8: 2234 (Journal)
- Registered Authors
- Chou, Shih-wei, Davis, Robin Woods, Fernando, Carol, McDermott Jr., Brian M., Zhu, Shaoyuan (Sara)
- Keywords
- none
- MeSH Terms
-
- Animals
- Lateral Line System/metabolism*
- Lateral Line System/physiology
- Mechanoreceptors/metabolism*
- Mechanoreceptors/physiology
- Mechanotransduction, Cellular/genetics*
- Mechanotransduction, Cellular/physiology
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Membrane Proteins/physiology
- Motion*
- Water*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/physiology
- PubMed
- 29269857 Full text @ Nat. Commun.
Citation
Chou, S.W., Chen, Z., Zhu, S., Davis, R.W., Hu, J., Liu, L., Fernando, C.A., Kindig, K., Brown, W.C., Stepanyan, R., McDermott, B.M. (2017) A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish. Nature communications. 8:2234.
Abstract
Detection of water motion by the lateral line relies on mechanotransduction complexes at stereocilia tips. This sensory system is comprised of neuromasts, patches of hair cells with stereociliary bundles arranged with morphological mirror symmetry that are mechanically responsive to two opposing directions. Here, we find that transmembrane channel-like 2b (Tmc2b) is differentially required for mechanotransduction in the zebrafish lateral line. Despite similarities in neuromast hair cell morphology, three classes of these cells can be distinguished by their Tmc2b reliance. We map mechanosensitivity along the lateral line using imaging and electrophysiology to determine that a hair cell's Tmc2b dependence is governed by neuromast topological position and hair bundle orientation. Overall, water flow is detected by molecular machinery that can vary between hair cells of different neuromasts. Moreover, hair cells within the same neuromast can break morphologic symmetry of the sensory organ at the stereocilia tips.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping