PUBLICATION
Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish
- Authors
- Endoh, M., Fujimoto, T., Yamaha, E., Arai, K.
- ID
- ZDB-PUB-171221-7
- Date
- 2017
- Source
- Zebrafish 15(1): 33-44 (Journal)
- Registered Authors
- Keywords
- developmental biology, embryogenesis, genetics
- MeSH Terms
-
- Animals
- Cryopreservation/methods*
- Female
- Fertilization
- Haploidy*
- Male
- Ovum
- Spermatozoa
- Ultraviolet Rays*
- Zebrafish/genetics*
- Zebrafish/physiology*
- PubMed
- 29261026 Full text @ Zebrafish
Citation
Endoh, M., Fujimoto, T., Yamaha, E., Arai, K. (2017) Improved Procedure for Induction of the Androgenetic Doubled Haploids in Zebrafish. Zebrafish. 15(1):33-44.
Abstract
Androgenesis is useful for induction of doubled haploids from male genetic resources and contributes to the restoration of individuals from cryopreserved sperm. Here, we determined the suitable conditions for egg in vitro preservation and the suitable dose of UV irradiation for genetic inactivation of the egg nucleus, and established an improved procedure for induction of androgenetic-doubled haploids in zebrafish. The suitable solution for egg preservation was evaluated by the fertilization rate using different types of solutions or conditions. Hank's solution with 0.5% bovine serum albumin (pH8.0) was suitable for the preservation of zebrafish eggs. In addition, we discovered an improvement of fertilization rates by temporal preservation of ovulated eggs in the suitable solution. UV irradiation of eggs at 50-75 mJ/cm2 induced haploid embryos. Microsatellite genotyping using eight loci revealed the paternity and homozygosity of the putative androgenetic doubled haploids. The yield rate of androgenetic doubled haploids, which were induced by UV irradiation and heat shock, ranged from 0.4% to 10.7%.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping