PUBLICATION

microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish

Authors
Kim, C.W., Han, J.H., Wu, L., Choi, J.Y.
ID
ZDB-PUB-171208-3
Date
2018
Source
Yonsei medical journal   59: 141-147 (Journal)
Registered Authors
Keywords
microRNA, ototoxicity, regeneration, zebrafish
MeSH Terms
  • Animals
  • Neomycin/toxicity
  • Zebrafish/genetics*
  • Gene Expression Profiling
  • Gene Expression Regulation/drug effects
  • Gene Knockdown Techniques
  • Hair Cells, Auditory/drug effects
  • Hair Cells, Auditory/physiology*
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Morpholinos/pharmacology
  • Cell Count
  • Animals, Genetically Modified
  • Larva/drug effects
  • Larva/genetics
  • Green Fluorescent Proteins/metabolism
  • Regeneration/drug effects
  • Regeneration/genetics*
(all 18)
PubMed
29214789 Full text @ Yonsei Med. J.
Abstract
Purpose microRNAs (miRNAs) are non-coding RNAs composed of 20 to 22 nucleotides that regulate development and differentiation in various organs by silencing specific RNAs and regulating gene expression. In the present study, we show that the microRNA (miR)-183 cluster is upregulated during hair cell regeneration and that its inhibition reduces hair cell regeneration following neomycin-induced ototoxicity in zebrafish.
Materials and methods miRNA expression patterns after neomycin exposure were analyzed using microarray chips. Quantitative polymerase chain reaction was performed to validate miR-183 cluster expression patterns following neomycin exposure (500 ?M for 2 h). After injection of an antisense morpholino (MO) to miR-183 (MO-183) immediately after fertilization, hair cell regeneration after neomycin exposure in neuromast cells was evaluated by fluorescent staining (YO-PRO1). The MO-183 effect also was assessed in transgenic zebrafish larvae expressing green fluorescent protein (GFP) in inner ear hair cells.
Results Microarray analysis clearly showed that the miR-183 cluster (miR-96, miR-182, and miR-183) was upregulated after neomycin treatment. We also confirmed upregulated expression of the miR-183 cluster during hair cell regeneration after neomycin-induced ototoxicity. miR-183 inhibition using MO-183 reduced hair cell regeneration in both wild-type and GFP transgenic zebrafish larvae.
Conclusion Our work demonstrates that the miR-183 cluster is essential for the regeneration of hair cells following ototoxic injury in zebrafish larvae. Therefore, regulation of the miR-183 cluster can be a novel target for stimulation of hair cell regeneration.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment by environment: neomycinProtruding-mouth
ABchemical treatment by environment: neomycinProtruding-mouth
ABchemical treatment by environment: neomycinProtruding-mouth
ABchemical treatment by environment: neomycinProtruding-mouth
ABchemical treatment by environment: neomycinDay 4
AB + MO1-dre-mir-183standard conditionsDay 5
AB + MO1-dre-mir-183chemical treatment by environment: neomycinDay 4
AB + MO3-dre-mir-182chemical treatment by environment: neomycinDay 4
s356tTgchemical treatment by environment: neomycinDay 4
s356tTgchemical treatment by environment: neomycinDay 4
1 - 10 of 12
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s356tTgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    Human Disease Fish Conditions Evidence
    sensorineural hearing lossTAS
    1 - 1 of 1
    Show
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    dre-mir-182MO3-dre-mir-182MRPHLNO
    dre-mir-183MO1-dre-mir-183MRPHLNO
    1 - 2 of 2
    Show
    Fish
    Fish
    AB
    AB + MO1-dre-mir-183
    AB + MO3-dre-mir-182
    s356tTg
    s356tTg + MO1-dre-mir-183
    1 - 5 of 5
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Kim, C.W., Han, J.H., Wu, L., Choi, J.Y. (2018) microRNA-183 is Essential for Hair Cell Regeneration after Neomycin Injury in Zebrafish. Yonsei medical journal. 59:141-147.