ZFIN ID: ZDB-PUB-171205-3
Evaluating estrogenic and anti-estrogenic effect of endocrine disrupting chemicals (EDCs) by zebrafish (Danio rerio) embryo-based vitellogenin 1 (vtg1) mRNA expression
Chen, M., Zhang, J., Pang, S., Wang, C., Wang, L., Sun, Y., Song, M., Liang, Y.
Date: 2017
Source: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   204: 45-50 (Journal)
Registered Authors: Chen, Minjie, Liang, Yong, Pang, Shaochen, Sun, Yonghua
Keywords: Endocrine disrupting chemicals, Estrogenic effect, Vitellogenin 1, Zebrafish embryo
MeSH Terms:
  • Animals
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism
  • Endocrine Disruptors/toxicity*
  • Estradiol/pharmacology
  • Gene Expression Regulation, Developmental/drug effects*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Vitellogenins/genetics
  • Vitellogenins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 29199129 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
By measuring the vitellogenin 1 (vtg1) expression through quantitative PCR and in situ hybridization, we used the zebrafish embryo as an in vivo model to access the estrogenic or anti-estrogenic effects of several endocrine disrupting chemicals (EDCs), such as natural estrogen 17β-estradiol (E2), estriol (E3), synthetic hormones including diethylstilbestrol (DES), 4-octyl phenol (OP), bisphenol A (BPA), tamoxifen (TMX) and 3-(2,3-dibromopropyl) isocyanurate (TBC). According to our data, the estrogenic effect of the tested chemicals was ranked as: DES>E2>E3>OP>BPA, which is consistent with various in vivo and in vitro models. Therefore, the measurement of vtg1 gene expression in zebrafish embryos would be a valuable method for screening EDCs including both environmental estrogens and anti-estrogens.