PUBLICATION
microRNA-145 regulates the RLR signaling pathway in miiuy croaker after poly(I:C) stimulation via targeting MDA5
- Authors
- Han, J., Sun, Y., Song, W., Xu, T.
- ID
- ZDB-PUB-171204-52
- Date
- 2017
- Source
- Developmental and comparative immunology 68: 79-86 (Journal)
- Registered Authors
- Keywords
- MDA5, MicroRNAs, Negatively regulate, RLRs, microRNA-145
- MeSH Terms
-
- DEAD-box RNA Helicases/genetics
- DEAD-box RNA Helicases/metabolism*
- Signal Transduction
- DEAD Box Protein 58/genetics
- DEAD Box Protein 58/metabolism*
- Computational Biology
- Computer Simulation
- Perciformes/immunology*
- MicroRNAs/genetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Gene Regulatory Networks/genetics*
- Liver/metabolism*
- Poly I-C/immunology
- Kidney/metabolism*
- Gene Expression
- Cells, Cultured
- Animals
- PubMed
- 27894672 Full text @ Dev. Comp. Immunol.
Citation
Han, J., Sun, Y., Song, W., Xu, T. (2017) microRNA-145 regulates the RLR signaling pathway in miiuy croaker after poly(I:C) stimulation via targeting MDA5. Developmental and comparative immunology. 68:79-86.
Abstract
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via degrading the target mRNAs or repressing translation. In this study, the regulation of miRNA to the RLR (RIG-I-like receptor) signaling pathway by degrading the target mRNAs was researched in miiuy croaker. MDA5, a microRNA-145-5p (miR-145-5p) putative target gene, was predicted by bioinformatics, and the target sites from the 3'untranslated region of MDA5 transcripts were confirmed using luciferase reporter assays. Pre-miR-145 was more effective in inhibiting MDA5 than miR-145-5p mimic, and the effect was dose- and time-dependent. The expression patterns of miR-145-5p and MDA5 were analyzed in liver and kidney from miiuy croaker. Results implied that miR-145-5p may function via degrading the MDA5 mRNAs, thereby regulating the RLR signaling pathway. Studies on miR-145-5p will enrich knowledge of its functions in immune response regulation in fish, as well as offer a basis for regulatory networks that are composed of numerous miRNAs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping