PUBLICATION
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
- Authors
- Conedera, F.M., Arendt, P., Trepp, C., Tschopp, M., Enzmann, V.
- ID
- ZDB-PUB-171121-9
- Date
- 2017
- Source
- Journal of visualized experiments : JoVE (128): (Journal)
- Registered Authors
- Tschopp, Markus
- Keywords
- none
- MeSH Terms
-
- Animals
- Cross-Sectional Studies
- Ependymoglial Cells/microbiology*
- Lasers
- Regeneration/physiology*
- Retinal Degeneration/metabolism*
- Retinal Degeneration/pathology
- Tomography, Optical Coherence/methods*
- Zebrafish
- PubMed
- 29155720 Full text @ J. Vis. Exp.
Citation
Conedera, F.M., Arendt, P., Trepp, C., Tschopp, M., Enzmann, V. (2017) Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish. Journal of visualized experiments : JoVE. (128).
Abstract
A fascinating difference between teleost and mammals is the lifelong potential of the teleost retina for retinal neurogenesis and regeneration after severe damage. Investigating the regeneration pathways in zebrafish might bring new insights to develop innovative strategies for the treatment of retinal degenerative diseases in mammals. Herein, we focused on the induction of a focal lesion to the outer retina in adult zebrafish by means of a 532 nm diode laser. A localized injury allows investigating biological processes that take place during retinal degeneration and regeneration directly at the area of damage. Using non-invasive optical coherence tomography (OCT), we were able to define the location of the damaged area and monitor subsequent regeneration in vivo. Indeed, OCT imaging produces high-resolution, cross-sectional images of the zebrafish retina, providing information which was previously only available with histological analyses. In order to confirm the data from real-time OCT, histological sections were performed and regenerative response after the induction of the retinal injury was investigated by immunohistochemistry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping