PUBLICATION

Evolution of Vertebrate Ryanodine Receptors Family in Relation to Functional Divergence and Conservation

Authors
Ding, Z., Peng, J., Liang, Y., Yang, C., Jiang, G., Ren, J., Zou, Y.
ID
ZDB-PUB-171121-2
Date
2017
Source
International heart journal   58(6): 969-977 (Journal)
Registered Authors
Keywords
Ancestral reconstruction, Motifs analysis, Mutation site, Phosphorylation site, Phylogenetic relationship, Positive selection
MeSH Terms
  • Animals
  • Chickens
  • Evolution, Molecular*
  • Gene Ontology
  • Humans
  • Lizards
  • Mice
  • Mutation
  • Phylogeny*
  • Rana catesbeiana
  • Rats
  • Ryanodine Receptor Calcium Release Channel/genetics*
  • Selection, Genetic
  • Zebrafish
PubMed
29151485 Full text @ Int Heart J
Abstract
Ryanodine receptors (RyRs), the large homotetrameric protein complexes, regulate the release of calcium from intracellular stores into the cytosol and play vital roles in the excitation-contraction coupling of cells. However, the evolutionary relationship of RyRs in vertebrates has yet to be elucidated. We identified 22 RyRs from Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Anolis carolinensis, Rana catesbeiana, and Danio rerio. The phylogenetic relationship, motifs analysis and reconstruction of ancestral RyRs showed that the members of RyR family in vertebrates were grouped into three clades: the RyR1 clade, the RyR2 clade, and the RyR3 clade. Positive selection existed in RyR gene evolution, which is consistent in three site models, and gene ontology (GO) analysis showed that the evolution of RyR family in vertebrates promotes RyRs function differentiation. At last, we predicted 140 mutation sites which may be involved in diseases and 57 phosphorylation sites among RyR1 sequence in human, as well as 61 mutation sites and 70 phosphorylation sites in human RyR2 sequences. Most of these potential sites are arranged in clusters. Our work provides insight into the origin and evolutionary process of RyRs in vertebrates, facilitating their functional investigations in the future.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping