Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
- Mateos, J.M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S.C.F., Kaech, A., Bachmann-Gagescu, R., Ziegler, U.
- Journal of visualized experiments : JoVE (129): (Journal)
- Registered Authors
- Bachmann-Gagescu, Ruxandra, Neuhauss, Stephan
- MeSH Terms
- Microscopy, Electron, Scanning/methods*
- Microscopy, Fluorescence/methods*
- Retina/diagnostic imaging*
- 29155784 Full text @ J. Vis. Exp.
Mateos, J.M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S.C.F., Kaech, A., Bachmann-Gagescu, R., Ziegler, U. (2017) Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina. Journal of visualized experiments : JoVE. (129).
We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of super-resolution light microscopes allow improving the accuracy of the correlated data. Briefly, 110 nanometer thick cryo-sections are transferred to a silicon wafer and, after immunofluorescence staining, are imaged by super-resolution light microscopy. Subsequently, the sections are preserved in methylcellulose and platinum shadowed prior to imaging in a scanning electron microscope (SEM). The images from these two microscopy modalities are easily merged using tissue landmarks with open source software. Here we describe the adapted method for the larval zebrafish retina. However, this method is also applicable to other types of tissues and organisms. We demonstrate that the complementary information obtained by this correlation is able to resolve the expression of mitochondrial proteins in relation with the membranes and cristae of mitochondria as well as to other compartments of the cell.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes