PUBLICATION

Transcriptional inhibition of TCDD-mediated induction of cytochrome P450 1A1 and alteration of protein expression in a zebrafish hepatic cell line following the administration of TCDD and Cd2.

Authors
Chen, Y.Y., Chan, K.M.
ID
ZDB-PUB-171107-7
Date
2017
Source
Toxicology letters   282: 121-135 (Journal)
Registered Authors
Chan, King-Ming
Keywords
Aryl hydrocarbon receptor, Gene transcription, Proteomics
MeSH Terms
  • Dose-Response Relationship, Drug
  • Environmental Pollutants/toxicity*
  • Liver/drug effects*
  • Liver/enzymology
  • Zebrafish
  • Animals
  • Zebrafish Proteins/genetics
  • Enzyme Induction
  • Cell Survival/drug effects
  • Cell Line
  • Cadmium Chloride/toxicity*
  • Gene Expression/drug effects*
  • Polychlorinated Dibenzodioxins/toxicity*
  • Cytochrome P-450 CYP1A1/biosynthesis*
(all 14)
PubMed
29107029 Full text @ Toxicol. Lett.
CTD
29107029
Abstract
We studied the effects of Cd2+ on TCDD-mediated induction of the cytochrome P450 1A1 (cyp1a1) gene using a zebrafish liver cell line (ZFL). Our results showed that Cd2+ inhibited the TCDD-mediated induction of the cyp1a1 protein, enzyme activity, and mRNA expression level. Cd2+ also down-regulated levels of the aryl hydrocarbon receptor (ahr2) and the aryl hydrocarbon receptor nuclear translocator 2b (arnt2b) mRNAs. Compared with TCDD (3nM) treatment alone, co-treatment with Cd2+ (0-30μM) and TCDD (3nM) significantly inhibited the activity of the luciferase reporter gene constructs harboring the distal promoter region (P-2626/-2009) of CYP1A1 and the synthetic 3XRE gene promoter. This indicates that Cd2+ decreased the level of TCDD-induced cyp1a1 through transcriptional inhibition. Proteomic analysis was also used to evaluate the effect of Cd2+ on TCDD-altered protein expression in ZFL cells. The identified proteins are mainly enzymes of the glycolysis pathway and proteasomes, and have anti-oxidative and anti-stress effects.
Genes / Markers
Figures
No images available
Expression
No data available
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping
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Citation
Chen, Y.Y., Chan, K.M. (2017) Transcriptional inhibition of TCDD-mediated induction of cytochrome P450 1A1 and alteration of protein expression in a zebrafish hepatic cell line following the administration of TCDD and Cd2.. Toxicology letters. 282:121-135.