PUBLICATION

Structure?function study of gemini derivatives with two different side chains at C-20, Gemini-0072 and Gemini-0097

Authors
Huet, T., Maehr, H., Lee, H.J., Uskokovic, M.R., Suh, N., Moras, D., Rochel, N.
ID
ZDB-PUB-171107-16
Date
2011
Source
MedChemComm   2(5): 424-429 (Journal)
Registered Authors
Keywords
none
MeSH Terms
none
PubMed
22180837 Full text @ MedChemComm
Abstract

Derivatives of vitamin D3 containing a second side-chain emanating at C-20 are known as gemini and act as vitamin D receptor agonists. Recently, two of these, namely Gemini-0072 and the epimeric Gemini-0097, were selected for further studies in view of their high biological activities and lack of hypercalcemic effects. We now show that the two analogs recruit coactivator SRC-1 better than the parental gemini and act as VDR superagonists. The crystal structures of complexes of zVDR with Gemini-0072 and Gemini-0097 indicate that these ligands induce an extra cavity within the ligand-binding pocket similar to gemini and that their superagonistic activity is due to an increased stabilization of helix H12.

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