PUBLICATION
Ancestral Circuits for the Coordinated Modulation of Brain State
- Authors
- Lovett-Barron, M., Andalman, A.S., Allen, W.E., Vesuna, S., Kauvar, I., Burns, V.M., Deisseroth, K.
- ID
- ZDB-PUB-171107-15
- Date
- 2017
- Source
- Cell 171(6): 1411-1423.e17 (Journal)
- Registered Authors
- Keywords
- Brain state, Calcium imaging, Mice, Neuromodulation, Volume registration, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal*
- Brain/cytology*
- Brain/physiology*
- Brain Mapping
- Larva/cytology
- Larva/physiology
- Mice
- Neural Pathways
- Neurons/cytology*
- Zebrafish/growth & development
- Zebrafish/physiology
- PubMed
- 29103613 Full text @ Cell
Citation
Lovett-Barron, M., Andalman, A.S., Allen, W.E., Vesuna, S., Kauvar, I., Burns, V.M., Deisseroth, K. (2017) Ancestral Circuits for the Coordinated Modulation of Brain State. Cell. 171(6):1411-1423.e17.
Abstract
Internal states of the brain profoundly influence behavior. Fluctuating states such as alertness can be governed by neuromodulation, but the underlying mechanisms and cell types involved are not fully understood. We developed a method to globally screen for cell types involved in behavior by integrating brain-wide activity imaging with high-content molecular phenotyping and volume registration at cellular resolution. We used this method (MultiMAP) to record from 22 neuromodulatory cell types in behaving zebrafish during a reaction-time task that reports alertness. We identified multiple monoaminergic, cholinergic, and peptidergic cell types linked to alertness and found that activity in these cell types was mutually correlated during heightened alertness. We next recorded from and controlled homologous neuromodulatory cells in mice; alertness-related cell-type dynamics exhibited striking evolutionary conservation and modulated behavior similarly. These experiments establish a method for unbiased discovery of cellular elements underlying behavior and reveal an evolutionarily conserved set of diverse neuromodulatory systems that collectively govern internal state.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping