PUBLICATION
Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio)
- Authors
- Zhang, C.N., Zhang, J.L., Huang, Y., Ren, H.T., Guan, S.H., Zeng, Q.H.
- ID
- ZDB-PUB-171101-3
- Date
- 2017
- Source
- Environmental toxicology 33(1): 104-111 (Journal)
- Registered Authors
- Keywords
- cytokines, dibutyltin (DBT), gene expression, immunity, zebrafish
- MeSH Terms
-
- Janus Kinase 2/genetics
- Janus Kinase 2/metabolism
- Interleukin-8/genetics
- Interleukin-8/metabolism
- Organotin Compounds/toxicity*
- I-kappa B Kinase/genetics
- I-kappa B Kinase/metabolism
- Signal Transduction/drug effects*
- Zebrafish/immunology
- Zebrafish/metabolism*
- Interleukin-6/genetics
- Interleukin-6/metabolism
- Interleukin-1beta/genetics
- Interleukin-1beta/metabolism
- STAT3 Transcription Factor/genetics
- STAT3 Transcription Factor/metabolism
- Transcription Factor RelA/genetics
- Transcription Factor RelA/metabolism
- NF-KappaB Inhibitor alpha/genetics
- NF-KappaB Inhibitor alpha/metabolism
- Intestines/drug effects
- Intestines/immunology
- Intestines/metabolism
- Animals
- Tumor Necrosis Factor-alpha/genetics
- Tumor Necrosis Factor-alpha/metabolism
- Skin/drug effects
- Skin/immunology
- Skin/metabolism
- Spleen/drug effects
- Spleen/immunology
- Spleen/metabolism
- PubMed
- 29087020 Full text @ Env. Tox.
Citation
Zhang, C.N., Zhang, J.L., Huang, Y., Ren, H.T., Guan, S.H., Zeng, Q.H. (2017) Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio). Environmental toxicology. 33(1):104-111.
Abstract
Dibutyltin (DBT) is the degradation products of TBT, which is generally considered higher toxicity than TBT in the immune system. In order to learn more about the mechanisms of immune-toxic of DBT, we exposed zebrafish (Danio rerio) to 0, 1, 10 and 100 ng/L DBT for 8 weeks. At the end of the experiment, we determined the immune parameters and immune-related genes. The results showed that with an increase in TBT dose, lysozyme activities and IgM, C3, C4 content in intestine, skin and spleen were all significantly inhibited by the DBT exposure. Fish exposed to 10 ng/L and 100 ng/L showed significantly lower lysozyme activities and IgM, C3, C4 content than those of the control group. Zebrafish exposed to 10 ng/L and 100 ng/L DBT, the mRNA transcript levels of interleukin-1? (IL-1?), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-? (TNF-?), interferon ?2 (INF?2), nuclear factor-?B p65 (NF-kB p65), inhibitor protein-?B? (I?B?), I?B kinases ? (IKK?), Janus family of protein tyrosine kinases (JAKs) and the signal transducers and activators of transcription proteins (STATs) all increased with the DBT levels in the intestine and spleen. Those parameters showed significantly higher values in 10 ng/L and 100 ng/L than those of fish in the control group. However, no significant difference was found in I?B kinases ? (IKK?) and I?B kinase ? (IKK?) mRNA levels in the intestine and spleen. These data imply that DBT might be via suppression on IKK?/IkBa/NF-kBp65 and JAK/STAT signaling pathways to regulate the immunity of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping