High-Throughput Automated Chemical Screens in Zebrafish
- Saydmohammed, M., Tsang, M.
- Methods in molecular biology (Clifton, N.J.) 1683: 383-393 (Chapter)
- Registered Authors
- Tsang, Michael
- Chemical genetics, Drug discovery, FGF hyper-activators, Small molecule screens, Transgenic reporter
- MeSH Terms
- Animals, Genetically Modified
- Drug Discovery*/methods
- Embryo, Nonmammalian/drug effects
- Gene Expression
- Genes, Reporter
- High-Throughput Screening Assays*
- Microscopy, Fluorescence
- Small Molecule Libraries
- 29082504 Full text @ Meth. Mol. Biol.
Saydmohammed, M., Tsang, M. (2018) High-Throughput Automated Chemical Screens in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1683:383-393.
Zebrafish are increasingly used to perform phenotypic screens to identify agents that can alter physiology in a whole organismal context. Here, we describe an automated high-content chemical screen using transgenic zebrafish embryos to identify small molecules that modulate Fibroblast Growth Factor Signaling. High content multi-well screening was further refined with a particular emphasis on automated imaging and quantification that increases sensitivity and throughput of whole organism chemical screens.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes