PUBLICATION

[Genetic manipulation in zebrafish]

Authors
Gao, Y., Liu, J., Wang, X., Liu, D.
ID
ZDB-PUB-171031-4
Date
2017
Source
Sheng wu gong cheng xue bao = Chinese journal of biotechnology   33: 1674-1692 (Journal)
Registered Authors
Gao, Yu, Liu, Dong, Wang, Xin
Keywords
gene editing, genetic manipulation, mutation, transgenic, transposon, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified*
  • CRISPR-Cas Systems*
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Endonucleases
  • Genetic Engineering*
  • Genome
  • Genomics
  • Mutagenesis*
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed
29082716 Full text @ Sheng Wu Gong Cheng Xue Bao
Abstract
The increasing number of genetic manipulation approaches and high-resolution live imaging technique applied in zebrafish have propelled the rise of this organism as a mainstream model for developmental biology and human diseases studies. Zebrafish has many advantages for functional genomics analysis, allowing for easy, cheap and fast functional characterization of novel genes in the vertebrate genome. Here we provide an overview of the principles of genetic manipulation in zebrafish, such as Ethylnitrosourea (ENU) mutagenesis, insertional mutagenesis, gene trapping mutagenesis, Morpholino mediated gene knockdown, targeting induced local lesions in genomes (TILLING), genome editing with engineered nucleases ZFN (Zinc finger nuclease), TALEN (Transcription activator-like effector nuclease) and CRISPR/Cas9 system, and transgenic methods used in zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes
[Article in Chinese; Abstract available in Chinese from the publisher]