PUBLICATION
Heat-induced oxidative stress and inflammation involve in cadmium pollution history in the spleen of zebrafish
- Authors
- Zhao, S.J., Guo, S.N., Zhu, Q.L., Yuan, S.S., Zheng, J.L.
- ID
- ZDB-PUB-171028-12
- Date
- 2017
- Source
- Fish & shellfish immunology 72: 1-8 (Journal)
- Registered Authors
- Keywords
- Cadmium pollution, Fish, Heat stress, Inflammation, Stress response
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Cadmium/metabolism*
- Dose-Response Relationship, Drug
- Female
- Hot Temperature/adverse effects*
- Immunity, Innate*
- Male
- Oxidative Stress*
- Water Pollutants, Chemical/metabolism*
- Zebrafish/metabolism*
- PubMed
- 29074130 Full text @ Fish Shellfish Immunol.
Citation
Zhao, S.J., Guo, S.N., Zhu, Q.L., Yuan, S.S., Zheng, J.L. (2017) Heat-induced oxidative stress and inflammation involve in cadmium pollution history in the spleen of zebrafish. Fish & shellfish immunology. 72:1-8.
Abstract
Zebrafish were exposed to 0, 2.5 and 5 μg/L cadmium (Cd) for 10 weeks, and then each group was exposed to 26 °C(control) and 32 °C (high temperature) for 7 days. 22 indicators were compared between 26 °C and 32 °C in the spleen, including body weight, LPO and NO levels, activity levels of Cu/Zn-SOD, CAT and iNOS, MTs protein levels, and mRNA levels of Nrf2, Cu/Zn-SOD, CAT, HSF1, HSF2, HSP70, MTF-1, MTs, IL-6, IL-10, IL-1β, TNF-α, iNOS and NF-κB. Most indicators were not significantly affected by heat in fish from no Cd pollution. However, almost all of indicators were responsive to heat in fish pre-exposed to Cd. Several indicators were sensitive to heat in fish pre-exposed to 2.5 μg/L Cd such as iNOS activities, and mRNA levels of iNOS and IL-10. Most other indicators were sensitive to heat in fish pre-exposed to 5 μg/L. The mRNA levels of HSP70 and MTF-1 were up-regulated by heat in fish pre-exposed to 0, 2.5 and 5 μg/L Cd. However, the magnitude of increase was the greatest in fish pre-exposed to 5 μg/L Cd. These differences between control and high temperature would serve as biomarkers to distinguish healthy from Cd-polluted group. The findings imply that metal pollution history should be carefully considered when screening heat biomarkers in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping