PUBLICATION
Assessing the environmental quality of sediments from Split coastal area (Croatia) with a battery of cell-based bioassays
- Authors
- Blanco, M., Pérez-Albaladejo, E., Piña, B., Kušpilić, G., Milun, V., Lille-Langøy, R., Karlsen, O.A., Goksøyr, A., Porte, C.
- ID
- ZDB-PUB-171028-11
- Date
- 2017
- Source
- The Science of the total environment 624: 1640-1648 (Journal)
- Registered Authors
- Piña, Benjamin
- Keywords
- EROD, Estrogenic activity, Kaštela Bay, PLHC-1, Sediment, Zebrafish Pxr
- MeSH Terms
-
- Animals
- Biological Assay*
- Cell Line
- Croatia
- Environmental Monitoring*
- Fishes
- Geologic Sediments/chemistry*
- Water Pollutants, Chemical/analysis*
- Zebrafish
- PubMed
- 29074243 Full text @ Sci. Total Environ.
Citation
Blanco, M., Pérez-Albaladejo, E., Piña, B., Kušpilić, G., Milun, V., Lille-Langøy, R., Karlsen, O.A., Goksøyr, A., Porte, C. (2017) Assessing the environmental quality of sediments from Split coastal area (Croatia) with a battery of cell-based bioassays. The Science of the total environment. 624:1640-1648.
Abstract
A battery of cell-based bioassays, including PLHC-1 cells, zebrafish-Pxr-transfected COS-7 cells and estrogen receptor-recombinant yeast assay (ER-RYA), were applied to detect the presence of bioactive pollutants in sediments collected from Kaštela Bay and Brač Channel (Croatia). Exposure of PLHC-1 cells to the sediment extracts evidenced significant cytotoxicity and presence of CYP1A inducers in sediments collected in Kaštela Bay, near the industrial zone and cargo port of Split. Sediments from this area, which is highly contaminated with PCBs, HCB, DDTs and γ-HCH, also activated the zebrafish Pxr (zfPxr) reporter system. No evidence of estrogenicity was detected for any of the sediments extracts in the ER-RYA assay. Importantly, the battery of in vitro assays identified Kaštela Bay as the area with the higher anthropogenic impact, where sediment-bound pollutants could pose a risk to aquatic organisms. In contrast, sediments from the Brač Channel showed rather low response in the different bioassays.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping