PUBLICATION

Potent anti-melanogenic activity and favorable toxicity profile of selected 4-phenyl hydroxycoumarins in the zebrafish model and the computational molecular modeling studies

Authors
Veselinović, J.B., Veselinović, A.M., Ilic-Tomic, T., Davis, R., O'Connor, K., Pavic, A., Nikodinovic-Runic, J.
ID
ZDB-PUB-171019-11
Date
2017
Source
Bioorganic & Medicinal Chemistry   25(24): 6286-6296 (Journal)
Registered Authors
Keywords
4-Phenyl hydroxycoumarins, Melanogenesis, Molecular docking, Tyrosinase inhibitors, Zebrafish
MeSH Terms
  • Animals
  • Coumarins/chemistry
  • Coumarins/pharmacology*
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors/chemistry
  • Enzyme Inhibitors/pharmacology*
  • Melanocytes
  • Models, Animal*
  • Models, Molecular
  • Molecular Structure
  • Monophenol Monooxygenase/antagonists & inhibitors
  • Monophenol Monooxygenase/metabolism
  • Structure-Activity Relationship
  • Zebrafish
PubMed
29042224 Full text @ Bioorg. Med. Chem.
Abstract
7-Hydroxy-4-phenylcoumarin (7C) and 5,7-dihydroxy-4-phenylcoumarin (5,7C) have been evaluated as potential anti-melanogenic agents in the zebrafish (Danio rerio) model in comparison to commercially utilized depigmenting agents hydroquinone and kojic acid. 7C and 5,7C decreased the body pigmentation at 5µg/mL, while did not affect the embryos development and survival at doses ≤50µg/mL and ≤25µg/mL. Unlike hydroquinone and kojic acid, 4-phenyl hydroxycoumarins were no melanocytotoxic, showed no cardiotoxic side effects, neither caused neutropenia in zebrafish embryos, suggesting these compounds may present novel skin-whitening agents with improved pharmacological properties. Inhibition of tyrosinase was identified as the possible mode of anti-melanogenic action. Molecular docking studies using the homology model of human tyrosinase as well as adenylate cyclase revealed excellent correlation with experimentally obtained results.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping