PUBLICATION

An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate

Authors
Barone, V., Lang, M., Krens, S.F.G., Pradhan, S.J., Shamipour, S., Sako, K., Sikora, M., Guet, C.C., Heisenberg, C.P.
ID
ZDB-PUB-171017-7
Date
2017
Source
Developmental Cell   43(2): 198-211.e12 (Journal)
Registered Authors
Barone, Vanessa, Heisenberg, Carl-Philipp, Krens, S. F. Gabby, Sako, Keisuke
Keywords
Nodal signaling, cell differentiation, cell-cell adhesion, cell-fate specification, endoderm, gastrulation, gene regulatory network, kinetic proofreading, mesoderm, zebrafish
MeSH Terms
  • Morphogenesis/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Models, Theoretical
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Nodal Protein/genetics
  • Nodal Protein/metabolism
  • Gastrula/growth & development
  • Gastrula/metabolism*
  • Body Patterning
  • Gastrulation/physiology
  • Cell Differentiation
  • Signal Transduction
  • Gene Expression Regulation, Developmental
  • Cell Lineage*
  • Feedback, Physiological*
  • Embryonic Development
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Cell Communication*
  • Animals
(all 24)
PubMed
29033362 Full text @ Dev. Cell
Abstract
Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo.
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Allele Construct Type Affected Genomic Region
e120TgTransgenic Insertion
    s870TgTransgenic Insertion
      tz257
        		Point Mutation
        u302TgTransgenic Insertion
          vu119TgTransgenic Insertion
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            Target Reagent Reagent Type
            cdh1MO4-cdh1MRPHLNO
            sox32MO1-sox32MRPHLNO
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            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            RFPEFGRFP
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            Citation
            Barone, V., Lang, M., Krens, S.F.G., Pradhan, S.J., Shamipour, S., Sako, K., Sikora, M., Guet, C.C., Heisenberg, C.P. (2017) An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate. Developmental Cell. 43(2):198-211.e12.