PUBLICATION

Impaired AMPA receptor trafficking by a double knockout of zebrafish olfactomedin1a/b

Authors
Nakaya, N., Sultana, A., Tomarev, S.I.
ID
ZDB-PUB-171005-5
Date
2017
Source
Journal of neurochemistry   143(6): 635-644 (Journal)
Registered Authors
Keywords
AMPA receptor, Olfactomedin 1, lipid raft, palmitoylation, zebrafish
MeSH Terms
  • Extracellular Matrix Proteins/genetics*
  • Receptors, AMPA/metabolism*
  • Brain/metabolism
  • Protein Transport/genetics*
  • Glycoproteins/genetics*
  • Gene Knockout Techniques
  • Zebrafish
  • Animals
(all 8)
PubMed
28975619 Full text @ J. Neurochem.
Abstract
The olfm1a and olfm1b genes in zebrafish encode conserved secreted glycoproteins. These genes are preferentially expressed in the brain and retina starting from 16 h post-fertilization until adulthood. Functions of the Olfm1 gene is still unclear. Here, we produced and analyzed a null zebrafish mutant of both olfm1a and olfm1b genes (olfm1 null). olfm1 null fish were born at a normal Mendelian ratio and showed normal body shape and fertility as well as no visible defects from larval stages to adult. Olfm1 proteins were preferentially localized in the synaptosomes of the adult brain. Olfm1 co-immunoprecipitated with GluR2 and SNARE complexes indicating participation of Olfm1 in both pre- and post-synaptic events. Phosphorylation of GluR2 was not changed while palmitoylation of GluR2 was decreased in the brain synaptosomal membrane fraction of olfm1 null compared with wt fish. The levels of GluR2, SNAP25, flotillin1 and VAMP2 were markedly reduced in the synaptic microdomain of olfm1 null brain compared with wt. The internalization of GluR2 in retinal cells and the localization of VAMP2 in brain synaptosome were modified by olfm1 null mutation. This indicates that Olfm1 may regulate receptor trafficking from the intracellular compartments to the synaptic membrane microdomain, partly through the alteration of post-translational GluR2 modifications such as palmitoylation. Olfm1 may be considered a novel regulator of the composition and function of the AMPAR complex. This article is protected by copyright. All rights reserved.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sa157
    		Point Mutation
    sa221
      		Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      olfm1asa221/sa221; olfm1bsa157/sa157 (TL)
      TL
      1 - 2 of 2
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      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Nakaya, N., Sultana, A., Tomarev, S.I. (2017) Impaired AMPA receptor trafficking by a double knockout of zebrafish olfactomedin1a/b. Journal of neurochemistry. 143(6):635-644.