PUBLICATION
Cord and maternal sera from small neonates share dysfunctional lipoproteins with proatherogenic properties: Evidence for Barker's hypothesis
- Authors
- Kim, S.M., Lee, S.M., Kim, S.J., Kim, B.J., Shin, S., Kim, J.R., Cho, K.H.
- ID
- ZDB-PUB-171003-7
- Date
- 2017
- Source
- Journal of clinical lipidology 11(6): 1318-1328.e3 (Journal)
- Registered Authors
- Keywords
- Cholesteryl ester transfer protein (CETP), Embryo, Lipoprotein, Small for gestational age, Zebrafish
- MeSH Terms
-
- Animals
- Apolipoprotein A-I/blood
- Biomarkers/blood*
- Fetal Blood/metabolism
- Fetal Growth Retardation/blood*
- Fetal Growth Retardation/physiopathology
- Humans
- Infant, Newborn
- Lipoproteins/blood*
- Maternal-Fetal Relations
- Metabolic Diseases/blood*
- Metabolic Diseases/physiopathology
- Perinatal Mortality
- Zebrafish
- PubMed
- 28966080 Full text @ J Clin Lipidol
Citation
Kim, S.M., Lee, S.M., Kim, S.J., Kim, B.J., Shin, S., Kim, J.R., Cho, K.H. (2017) Cord and maternal sera from small neonates share dysfunctional lipoproteins with proatherogenic properties: Evidence for Barker's hypothesis. Journal of clinical lipidology. 11(6):1318-1328.e3.
Abstract
Background Fetal growth restriction (GR) is associated with perinatal mortality and subsequent metabolic disorders in adulthood. Until now, there is little information regarding changes in the properties of lipoproteins from growth-restricted fetuses and their maternal sera.
Objective To identify unique lipoprotein biomarkers for fetal GR in maternal and cord sera from small neonates, we analyzed lipoprotein compositions and functions.
Methods Lipoprotein compositions and functions were compared between cord blood and maternal blood among small for gestational age neonates (SGA; n = 15, 2589 ± 50 g) and appropriate for gestational age neonates (AGA; n = 15) in Korea.
Results Cord blood from the SGA group showed 2-fold higher triglyceride (TG) and TG/high-density lipoprotein cholesterol levels than the AGA group as well as significantly lower (up to 20%) paraoxonase activity and apolipoprotein (apo) A-I content. The SGA group showed the highest cholesteryl ester transfer protein activities in both cord and maternal sera. SGA neonates showed elevated apo-B content in very low-density lipoprotein, 52% reduction of apo A-I content in high-density lipoprotein, and 30% increased glycation (P < .001) compared with AGA neonates. Especially, low-density lipoprotein from the SGA group showed 1.9-fold higher sensitivity to oxidation as well as 3-fold greater uptake into macrophages, suggesting stronger proatherosclerotic properties. Lipoproteins from maternal serum of SGA neonates showed greater oxidation along with TG enrichment and loss of antioxidant ability. On microinjection of cord serum (50 nL) into zebrafish embryos, the SGA group showed the most severe embryonic damage.
Conclusions Lipoproteins from cord and maternal sera of SGA neonates resulted in severe impairment of functional and structural correlations accompanied by greater pro-oxidant and proatherosclerotic properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping