PUBLICATION

Ferulic Acid Promotes Hypertrophic Growth of Fast Skeletal Muscle in Zebrafish Model

Authors
Wen, Y., Ushio, H.
ID
ZDB-PUB-170929-6
Date
2017
Source
Nutrients   9(10): (Journal)
Registered Authors
Keywords
fast myofibers, ferulic acid, muscular hypertrophy, protein synthesis, sarcomeric unit, skeletal muscle mass
MeSH Terms
  • Muscle, Skeletal/drug effects*
  • Muscle, Skeletal/growth & development
  • Muscle, Skeletal/metabolism
  • Muscle Proteins/biosynthesis
  • Muscle Proteins/genetics
  • Coumaric Acids/pharmacology*
  • Male
  • Time Factors
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish
  • Models, Animal
  • Weight Gain/drug effects
  • Transcriptional Activation/drug effects
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics
  • Hypertrophy
  • Signal Transduction/drug effects
  • Muscle Fibers, Fast-Twitch/drug effects*
  • Muscle Fibers, Fast-Twitch/metabolism
  • Animals
  • Body Mass Index
  • Organ Size
  • Gene Expression Regulation/drug effects
(all 24)
PubMed
28954428 Full text @ Nutrients
Abstract
As a widely distributed and natural existing antioxidant, ferulic acid and its functions have been extensively studied in recent decades. In the present study, hypertrophic growth of fast skeletal myofibers was observed in adult zebrafish after ferulic acid administration for 30 days, being reflected in increased body weight, body mass index (BMI), and muscle mass, along with an enlarged cross-sectional area of myofibers. qRT-PCR analyses demonstrated the up-regulation of relative mRNA expression levels of myogenic transcriptional factors (MyoD, myogenin and serum response factor (SRF)) and their target genes encoding sarcomeric unit proteins involved in muscular hypertrophy (skeletal alpha-actin, myosin heavy chain, tropomyosin, and troponin I). Western blot analyses detected a higher phosphorylated level of zTOR (zebrafish target of rapamycin), p70S6K, and 4E-BP1, which suggests an enhanced translation efficiency and protein synthesis capacity of fast skeletal muscle myofibers. These changes in transcription and translation finally converge and lead to higher protein contents in myofibers, as confirmed by elevated levels of myosin heavy chain (MyHC), and an increased muscle mass. To the best of our knowledge, these findings have been reported for the first time in vivo and suggest potential applications of ferulic acid as functional food additives and dietary supplements owing to its ability to promote muscle growth.
Genes / Markers
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-eif4ebp1monoclonalIgGRabbit
Ab2-mymonoclonal
    IgG1Mouse
    Ab3-mtorpolyclonal
      Rabbit
      Ab4-mtorpolyclonal
        Rabbit
        Ab5-eif4ebp1polyclonal
          Rabbit
          Ab8-rps6kpolyclonal
            Rabbit
            Ab13-rps6kpolyclonal
              Rabbit
              1 - 7 of 7
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              Orthology
              No data available
              Engineered Foreign Genes
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              Mapping
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