PUBLICATION
Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models.
- Authors
- Al-Samadi, A., Awad, S.A., Tuomainen, K., Zhao, Y., Salem, A., Parikka, M., Salo, T.
- ID
- ZDB-PUB-170928-14
- Date
- 2017
- Source
- Oncotarget 8: 60123-60134 (Journal)
- Registered Authors
- Keywords
- cytotoxicity, extracellular vesicles, immune cells, in vitro model, tongue cancer
- MeSH Terms
- none
- PubMed
- 28947958 Full text @ Oncotarget
Citation
Al-Samadi, A., Awad, S.A., Tuomainen, K., Zhao, Y., Salem, A., Parikka, M., Salo, T. (2017) Crosstalk between tongue carcinoma cells, extracellular vesicles, and immune cells in in vitro and in vivo models.. Oncotarget. 8:60123-60134.
Abstract
The crosstalk between immune cells, cancer cells, and extracellular vesicles (EVs) secreted by cancer cells remains poorly understood. We created three-dimensional (3D) cell culture models using human leiomyoma discs and Myogel to study the effects of immune cells on highly (HSC-3) and less (SCC-25) invasive oral tongue squamous cell carcinoma (OTSCC) cell lines. Additionally, we studied the effects of EVs isolated from these cell lines on the cytotoxicity of CD8+ T and NK cells isolated from three healthy donors. Our analysis included the effects of these EVs on innate immunity in zebrafish larvae. Activated immune cells significantly decreased the proliferation of both OTSCC cell lines and associated with a diminished invasion area of HSC-3 cells. In general, EVs from SCC-25 increased the cytotoxic activity of CD8+ T and NK cells more than those from HSC-3 cells. However, this effect varied depending on the source and the immune and cancer cell subgroups. In zebrafish, the amount of IL-13 mRNA was decreased by SCC-25 EVs. This study describes promising in vitro and in vivo models to investigate interactions between immune cells, cancer cells, and EVs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping