PUBLICATION
Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation
- Authors
- Pei, W., Tanaka, K., Huang, S.C., Xu, L., Liu, B., Sinclair, J., Idol, J., Varshney, G.K., Huang, H., Lin, S., Nussenblatt, R.B., Mori, R., Burgess, S.M.
- ID
- ZDB-PUB-170926-14
- Date
- 2016
- Source
- NPJ Regenerative medicine 1: 16013 (Journal)
- Registered Authors
- Keywords
- Cell death and immune response, Genetic interaction, Regeneration
- MeSH Terms
- none
- PubMed
- 28936359 Full text @ NPJ Regen Med
Citation
Pei, W., Tanaka, K., Huang, S.C., Xu, L., Liu, B., Sinclair, J., Idol, J., Varshney, G.K., Huang, H., Lin, S., Nussenblatt, R.B., Mori, R., Burgess, S.M. (2016) Extracellular HSP60 triggers tissue regeneration and wound healing by regulating inflammation and cell proliferation. NPJ Regenerative medicine. 1:16013.
Abstract
After injury, zebrafish can restore many tissues that do not regenerate well in mammals, making it a useful vertebrate model for studying regenerative biology. We performed a systematic screen to identify genes essential for hair cell regeneration in zebrafish, and found that the heat shock protein Hspd1 (Hsp60) has a critical role in the regeneration of hair cells and amputated caudal fins. We showed HSP60-injected extracellularly promoted cell proliferation and regeneration in both hair cells and caudal fins. We showed that hspd1 mutant was deficient in leukocyte infiltration at the site of injury. Topical application of HSP60 in a diabetic mouse skin wound model dramatically accelerated wound healing compared with controls. Stimulation of human peripheral blood mononuclear cells with HSP60 triggered a specific induction of M2 phase CD163-positive monocytes. Our results demonstrate that the normally intracellular chaperonin HSP60 has an extracellular signalling function in injury inflammation and tissue regeneration, likely through promoting the M2 phase for macrophages.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping