PUBLICATION
Chimeric ZHHH neuroglobin acts as a cell membrane-penetrating inducer of neurite outgrowth
- Authors
- Takahashi, N., Onozuka, W., Watanabe, S., Wakasugi, K.
- ID
- ZDB-PUB-170915-7
- Date
- 2017
- Source
- FEBS Open Bio 7: 1338-1349 (Journal)
- Registered Authors
- Wakasugi, Keisuke
- Keywords
- cell membrane?penetrating protein, neurite outgrowth, neuroglobin, rat pheochromocytoma PC12 cell line
- MeSH Terms
- none
- PubMed
- 28904863 Full text @ FEBS Open Bio
Abstract
Neuroglobin (Ngb) is a heme protein expressed in the vertebrate brain. We previously engineered a chimeric Ngb protein, in which module M1 of human Ngb is replaced by that of zebrafish Ngb, and showed that the chimeric ZHHH Ngb has a cell membrane-penetrating activity similar to that of zebrafish Ngb and also rescues cells from death caused by hypoxia/reoxygenation as does human Ngb. Recently, it was reported that overexpression of mammalian Ngb in neuronal cells induces neurite outgrowth. In this study, we performed neurite outgrowth assays of chimeric Ngb using rat pheochromocytoma PC12 cells. Addition of chimeric Ngb, but not human or zebrafish Ngb, exogenously to the cell medium induces neurite outgrowth. On the other hand, the K7A/K9Q chimeric Ngb double mutant, which cannot translocate into cells, did not induce neurite outgrowth, suggesting that the cell membrane-penetrating activity of the chimeric Ngb is crucial for its neurite outgrowth-promoting activity. We also prepared several site-directed chimeric Ngb mutants and demonstrated that residues crucial for neurite outgrowth-inducing activity of the chimeric Ngb are not exactly the same as those for its neuroprotective activity.
Genes / Markers
Figure Gallery (2 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping