PUBLICATION
Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish
- Authors
- Abe, F.R., Gravato, C., Soares, A.M.V.M., de Oliveira, D.P.
- ID
- ZDB-PUB-170912-7
- Date
- 2017
- Source
- Journal of toxicology and environmental health. Part A 80(23-24): 1259-1268 (Journal)
- Registered Authors
- Soares, Amadeu
- Keywords
- none
- MeSH Terms
-
- Animals
- Antioxidants/metabolism
- Azo Compounds/toxicity*
- Biomarkers/metabolism
- Coloring Agents/toxicity*
- Embryo, Nonmammalian/metabolism
- Environmental Monitoring/methods*
- Oxidative Stress/drug effects*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/growth & development
- Zebrafish/metabolism*
- PubMed
- 28891787 Full text @ J. Toxicol. Environ. Health. A.
Citation
Abe, F.R., Gravato, C., Soares, A.M.V.M., de Oliveira, D.P. (2017) Biochemical approaches to assess oxidative stress induced by exposure to natural and synthetic dyes in early life stages in zebrafish. Journal of toxicology and environmental health. Part A. 80(23-24):1259-1268.
Abstract
Zebrafish early life stages were found to be sensitive to several synthetic dyes widely used in industries. However, as environmental concentrations of such contaminants are often at sublethal levels, more sensitive methods are required to determine early-warning adverse consequences. The aim of this study was to utilize a multibiomarker approach to examine underlying oxidative stress mechanisms triggered by sublethal concentrations of synthetic azo dye Basic Red 51 (BR51), the natural dye erythrostominone (ERY), and its light-degraded product using zebrafish embryos. Biochemical biomarkers included parameters of detoxification and markers of antioxidant system, as well as oxidative damage. Results showed pro-oxidant mechanisms attributed to BR51 and ERY as evidenced by increased glutathione S-transferase (GST) activity, a phase II detoxification enzyme related to reactive oxygen species detoxification. BR51 also elevated total glutathione (GSH+GSSG) levels and catalase activity. However, both dyes induced oxidative damage as evidenced by elevated lipid peroxidation content. In contrast, when the natural dye was photodegraded, no marked effects were observed for all biomarkers assessed. Data indicate that such dyes are pro-oxidants at sublethal concentrations, predominantly involving GSH and/or related enzymes pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping