PUBLICATION
Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy
- Authors
- Fernández, A., Grüner-Nielsen, L., Andreana, M., Stadler, M., Kirchberger, S., Sturtzel, C., Distel, M., Zhu, L., Kautek, W., Leitgeb, R., Baltuska, A., Jespersen, K., Verhoef, A.
- ID
- ZDB-PUB-170901-7
- Date
- 2017
- Source
- Biomedical Optics Express 8: 3526-3537 (Journal)
- Registered Authors
- Distel, Martin, Kirchberger, Stefanie, Stadler, Manuela, Sturtzel, Caterina
- Keywords
- Fiber optics amplifiers and oscillators, Nonlinear microscopy, Scanning microscopy, Ultrafast lasers
- MeSH Terms
- none
- PubMed
- 28856032 Full text @ Biomed. Opt. Express
Citation
Fernández, A., Grüner-Nielsen, L., Andreana, M., Stadler, M., Kirchberger, S., Sturtzel, C., Distel, M., Zhu, L., Kautek, W., Leitgeb, R., Baltuska, A., Jespersen, K., Verhoef, A. (2017) Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy. Biomedical Optics Express. 8:3526-3537.
Abstract
A simple and completely all-fiber Yb chirped pulse amplifier that uses a dispersion matched fiber stretcher and a spliced-on hollow core photonic bandgap fiber compressor is applied in nonlinear optical microscopy. This stretching-compression approach improves compressibility and helps to maximize the fluorescence signal in two-photon laser scanning microscopy as compared with approaches that use standard single mode fibers as stretcher. We also show that in femtosecond all-fiber systems, compensation of higher order dispersion terms is relevant even for pulses with relatively narrow bandwidths for applications relying on nonlinear optical effects. The completely all-fiber system was applied to image green fluorescent beads, a stained lily-of-the-valley root and rat-tail tendon. We also demonstrated in vivo imaging in zebrafish larvae, where we simultaneously measure second harmonic and fluorescence from two-photon excited red-fluorescent protein. Since the pulses are compressed in a fiber, this source is especially suited for upgrading existing laser scanning (confocal) microscopes with multiphoton imaging capabilities in space restricted settings or for incorporation in endoscope-based microscopy.
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