PUBLICATION
Semicarbazide disturbs the reproductive system of male zebrafish (Danio rerio) through the GABAergic system
- Authors
- Yu, M., Feng, Y., Zhang, X., Wang, J., Tian, H., Wang, W., Ru, S.
- ID
- ZDB-PUB-170824-3
- Date
- 2017
- Source
- Reproductive toxicology (Elmsford, N.Y.) 73: 149-157 (Journal)
- Registered Authors
- Keywords
- Gamma-aminobutyric acid, Male zebrafish, Mechanism, Reproductive endocrine disruption, Semicarbazide
- MeSH Terms
-
- Animals
- Brain/drug effects*
- Brain/metabolism
- Endocrine Disruptors/toxicity*
- Estradiol/blood
- Leptin/genetics
- Male
- Semicarbazides/toxicity*
- Testis/drug effects*
- Testis/metabolism
- Testis/pathology
- Testosterone/blood
- Transcriptome/drug effects
- Zebrafish
- gamma-Aminobutyric Acid/metabolism
- PubMed
- 28834696 Full text @ Reprod. Toxicol.
- CTD
- 28834696
Citation
Yu, M., Feng, Y., Zhang, X., Wang, J., Tian, H., Wang, W., Ru, S. (2017) Semicarbazide disturbs the reproductive system of male zebrafish (Danio rerio) through the GABAergic system. Reproductive toxicology (Elmsford, N.Y.). 73:149-157.
Abstract
Semicarbazide (SMC), an emerging water contaminant, exerts anti-estrogenic effects in female zebrafish. However, the exact influence of SMC on male reproduction remains unclear. In this study, adult male zebrafish were exposed to 1-1000μg/L SMC in a semi-static system for 28 d prior to examining the testicular somatic index (TSI), testis histology, plasma sex hormone levels, and the transcription of genes involved in reproduction. The results showed that testicular morphology was altered and TSI was down-regulated by high concentrations of SMC (≥100μg/L and 1000μg/L, respectively). Plasma testosterone and 17β-estradiol concentrations were significantly decreased by all of the SMC treatments, along with down-regulation of the corresponding steroidogenic gene transcripts. These changes were associated with the inhibition of gamma-aminobutyric acid synthesis and function, in addition to the decreased expression of reproductive regulators. Our results contribute to elucidating the mechanisms underlying the adverse reproductive effects of SMC in male zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping