PUBLICATION
EFFECTS OF BIFENTHRIN EXPOSURE ON THE ESTROGENIC AND DOPAMINERGIC PATHWAYS IN ZEBRAFISH EMBRYOS AND JUVENILES
- Authors
- Bertotto, L.B., Richards, J., Gan, J., Volz, D.C., Schlenk, D.
- ID
- ZDB-PUB-170818-9
- Date
- 2017
- Source
- Environmental toxicology and chemistry 37(1): 236-246 (Journal)
- Registered Authors
- Keywords
- Developmental Toxicity, Dopaminergic Pathway, Endocrine Disrupting Compounds, Estrogenic Pathway, Pyrethroids, Zebrafish
- MeSH Terms
-
- 3,4-Dihydroxyphenylacetic Acid/metabolism
- Animals
- Dopamine/metabolism*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Environmental Exposure/analysis*
- Estrogens/metabolism*
- Gene Expression Regulation, Developmental/drug effects
- Homovanillic Acid/metabolism
- Metabolome
- Organ Specificity
- Pyrethrins/toxicity*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Signal Transduction/drug effects
- Signal Transduction/genetics
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 28815728 Full text @ Environ. Toxicol. Chem.
Citation
Bertotto, L.B., Richards, J., Gan, J., Volz, D.C., Schlenk, D. (2017) EFFECTS OF BIFENTHRIN EXPOSURE ON THE ESTROGENIC AND DOPAMINERGIC PATHWAYS IN ZEBRAFISH EMBRYOS AND JUVENILES. Environmental toxicology and chemistry. 37(1):236-246.
Abstract
Bifenthrin (BF) is a pyrethroid insecticide used in urban and agricultural applications. Previous studies have shown that environmentally relevant (ng/L) concentrations of BF increased plasma concentrations of 17β-estradiol (E2) and altered the expression of dopaminergic (DA) pathway components. DA neurons can indirectly regulate E2 biosynthesis, suggesting that BF may disrupt the hypothalamic-pituitary-gonadal (HPG) axis. Since embryos do not have a complete HPG axis, the hypothesis that BF impairs DA regulation was tested in embryonic and one-month old juvenile zebrafish (Danio rerio) with exposure to measured concentrations of 0.34 and 3.1 µg/L BF for 96 hours. Transcripts of tyrosine hydroxylase (TH), dopamine receptor 1 (DR1) and 2A (DR2A), dopamine active transporter (DAT), estrogen receptor α (ERα), ERβ1, ERβ2, LHβ, FSHβ, vitellogenin (VTG), cytochrome P450 cyp19a1a and cyp19a1b were investigated by qRT-PCR. Levels of E2 were measured by ELISA. Dopamine and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) concentrations were measured by LC-MS/MS. Significant decreases of TH and DR1 transcripts, and HVA levels as well as ratios of HVA/DA and HVA + DOPAC/DA in zebrafish embryos were observed after BF treatment. In juveniles, a significant increase in the expression of ERβ1 and the DOPAC/DA ratio was noted. These results show a possible anti-estrogenic effect of BF in embryos, and estrogenicity in juveniles, indicating life-stage dependent toxicity in developing fish. This article is protected by copyright. All rights reserved.
Errata / Notes
This article is corrected by ZDB-PUB-220906-112.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping