PUBLICATION
Effects of 2,2',4,4'-tetrabromodiphenyl ether on neurobehavior and memory change and bcl-2, c-fos, grin1b and lingo1b gene expression in male zebrafish (Danio rerio)
- Authors
- Zheng, S., Liu, C., Huang, Y., Bao, M., Huang, Y., Wu, K.
- ID
- ZDB-PUB-170817-17
- Date
- 2017
- Source
- Toxicology and applied pharmacology 333: 10-16 (Journal)
- Registered Authors
- Wu, Kusheng
- Keywords
- Maze learning, Memory testing, Neurobehavioral changes, Neurotoxicity, PBDEs
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Dose-Response Relationship, Drug
- Flame Retardants/toxicity*
- Gene Expression Regulation/drug effects
- Halogenated Diphenyl Ethers/toxicity*
- Male
- Maze Learning/drug effects*
- Membrane Proteins/genetics
- Memory/drug effects*
- Nerve Tissue Proteins/genetics
- Proto-Oncogene Proteins c-bcl-2/genetics
- Proto-Oncogene Proteins c-fos/genetics
- Receptors, N-Methyl-D-Aspartate/genetics
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 28807763 Full text @ Tox. App. Pharmacol.
- CTD
- 28807763
Citation
Zheng, S., Liu, C., Huang, Y., Bao, M., Huang, Y., Wu, K. (2017) Effects of 2,2',4,4'-tetrabromodiphenyl ether on neurobehavior and memory change and bcl-2, c-fos, grin1b and lingo1b gene expression in male zebrafish (Danio rerio). Toxicology and applied pharmacology. 333:10-16.
Abstract
Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants in various environmental matrices and organisms and pose a threat to neural systems of organisms. However, though quite a few studies have explored the effect of PBDEs on neural behaviors such as learning and memory abilities in animals, their mechanisms are less known. We used the zebrafish model to evaluate neurotoxicity of PBDEs and observe changes in behavior and related gene expression. In behavioral testing, 50 zebrafish were divided into five groups treated with different concentrations of BDE-47. T-maze exploration was used for learning and memory testing, which was recorded by camera every 7days. After 21days, all fish were killed, and the gene expression of c-fos, bcl-2, lingo1b and grin1b in brain tissue was analyzed by RT-qPCR. The behavioral changes (latency to leave the start zone, reach the reward zone, and stay in the reward zone; accuracy in choosing the right maze arm, accumulation of freezing bouts, etc.) were related to BDE-47 concentration and had a time-effect relation with increasing exposure days, especially with 500μg/L BDE-47. BDE-47 elevated brain bcl-2, grin1b and lingo1b expression. The expression of c-fos showed an increase with 50 and 100μg/L BDE-47 exposure. The PBDE BDE-47 had a negative impact on the neurobehaviors of zebrafish and affected the expression of c-fos, bcl-2, lingo1b and grin1b in zebrafish brain tissue.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping