PUBLICATION

Heart morphogenesis gene regulatory networks revealed by temporal expression analysis

Authors
Hill, J.T., Demarest, B., Smith, M., Gorsi, B., Yost, H.J.
ID
ZDB-PUB-170817-15
Date
2017
Source
Development (Cambridge, England)   144(19): 3487-3498 (Journal)
Registered Authors
Demarest, Bradley, Yost, H. Joseph
Keywords
Gene regulatory network, Heart development, RNA-seq, Time course
MeSH Terms
  • Animals
  • Cluster Analysis
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Gene Regulatory Networks*
  • Genes, Duplicate
  • Heart/embryology*
  • Mice
  • Morphogenesis/genetics*
  • Mutation/genetics
  • Nucleotide Motifs/genetics
  • Organ Specificity/genetics
  • Protein Binding
  • Sequence Analysis, RNA
  • Time Factors
  • Transcription Factors/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
28807900 Full text @ Development
Abstract
During embryogenesis the heart forms as a linear tube that then undergoes multiple simultaneous morphogenetic events to obtain its mature shape. To understand the gene regulatory networks (GRNs) driving this phase of heart development, during which many congenital heart disease malformations likely arise, we conducted an RNA-seq timecourse in zebrafish from 30 hpf to 72 hpf and identified 5861 genes with altered expression. We clustered the genes by temporal expression pattern, identified transcription factor binding motifs enriched in each cluster, and generated a model GRN for the major gene batteries in heart morphogenesis. This approach predicted hundreds of regulatory interactions and found batteries enriched in specific cell and tissue types, indicating that the approach can be used to narrow the search for novel genetic markers and regulatory interactions. Subsequent analyses confirmed the GRN using two mutants, Tbx5 and nkx2-5, and identified sets of duplicated zebrafish genes that do not show temporal subfunctionalization. This dataset provides an essential resource for future studies on the genetic/epigenetic pathways implicated in congenital heart defects and the mechanisms of cardiac transcriptional regulation.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping