PUBLICATION
Analysis of neurobehavioural data by chemometric methods in ecotoxicological studies
- Authors
- Gómez-Canela, C., Prats, E., Tauler, R., Raldúa, D.
- ID
- ZDB-PUB-170813-1
- Date
- 2017
- Source
- Ecotoxicology and environmental safety 145: 583-590 (Journal)
- Registered Authors
- Raldúa, Demetrio
- Keywords
- Behaviour, Chemometric tools, Open field test, Zebrafish
- MeSH Terms
-
- Analysis of Variance
- Animals
- Behavior, Animal/drug effects*
- Chlorpyrifos/metabolism
- Chlorpyrifos/toxicity
- Ecotoxicology/methods*
- Ecotoxicology/statistics & numerical data
- Environmental Pollutants/metabolism
- Environmental Pollutants/toxicity*
- Motor Activity/drug effects*
- Nicotine/metabolism
- Nicotine/toxicity
- Principal Component Analysis
- Zebrafish/metabolism*
- PubMed
- 28802139 Full text @ Ecotoxicol. Environ. Saf.
Citation
Gómez-Canela, C., Prats, E., Tauler, R., Raldúa, D. (2017) Analysis of neurobehavioural data by chemometric methods in ecotoxicological studies. Ecotoxicology and environmental safety. 145:583-590.
Abstract
Incorporation of chemometric tools in behavioural data management workflows allows for the early identification of most relevant endpoints complementarily to statistical confirmatory approaches. In this work, the effects of two model neurotoxicants, chlorpyrifos (CPF) and nicotine, exposures on behavioural profiles of adult zebrafish at three different times (2, 6 and 24h) were evaluated using open field test (OFT) paradigm experiments. Two chemometric methods like Principal Component Analysis (PCA) and Analysis of Variance-Simultaneous Component Analysis (ASCA) have been used to interpret the changes observed in the obtained behavioural data. A decreased of the locomotor activity, an anxiolytic effect and an altered exploratory behaviour were the most affected behavioural endpoints in the CPF exposures. However, an increase of the locomotor activity and an anxiogenic effect were observed in the nicotine exposures. Finally, an excellent correlation between the ASCA results and the results obtained using traditional statistical procedures for both compounds were encountered.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping