PUBLICATION
Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience
- Authors
- Avitan, L., Pujic, Z., Mölter, J., Van De Poll, M., Sun, B., Teng, H., Amor, R., Scott, E.K., Goodhill, G.J.
- ID
- ZDB-PUB-170808-3
- Date
- 2017
- Source
- Current biology : CB 27(16): 2407-2419.e4 (Journal)
- Registered Authors
- Pujic, Zac, Scott, Ethan
- Keywords
- behavior, calcium imaging, critical period, dark rearing, graph theory, neural assemblies, neural coding, neural plasticity, prey capture, visual development
- MeSH Terms
-
- Zebrafish/growth & development
- Zebrafish/physiology*
- Female
- Animals
- Superior Colliculi/growth & development
- Superior Colliculi/physiology*
- Male
- Visual Pathways/growth & development
- Visual Pathways/physiology*
- PubMed
- 28781054 Full text @ Curr. Biol.
Citation
Avitan, L., Pujic, Z., Mölter, J., Van De Poll, M., Sun, B., Teng, H., Amor, R., Scott, E.K., Goodhill, G.J. (2017) Spontaneous Activity in the Zebrafish Tectum Reorganizes over Development and Is Influenced by Visual Experience. Current biology : CB. 27(16):2407-2419.e4.
Abstract
Spontaneous patterns of activity in the developing visual system may play an important role in shaping the brain for function. During the period 4-9 dpf (days post-fertilization), larval zebrafish learn to hunt prey, a behavior that is critically dependent on the optic tectum. However, how spontaneous activity develops in the tectum over this period and the effect of visual experience are unknown. Here we performed two-photon calcium imaging of GCaMP6s zebrafish larvae at all days from 4 to 9 dpf. Using recently developed graph theoretic techniques, we found significant changes in both single-cell and population activity characteristics over development. In particular, we identified days 5-6 as a critical moment in the reorganization of the underlying functional network. Altering visual experience early in development altered the statistics of tectal activity, and dark rearing also caused a long-lasting deficit in the ability to capture prey. Thus, tectal development is shaped by both intrinsic factors and visual experience.
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