PUBLICATION
Acute exposure to tris (2-butoxyethyl) phosphate (TBOEP) affects growth and development of embryo-larval zebrafish
- Authors
- Liu, Y., Wu, D., Xu, Q., Yu, L., Liu, C., Wang, J.
- ID
- ZDB-PUB-170805-12
- Date
- 2017
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 191: 17-24 (Journal)
- Registered Authors
- Keywords
- Growth hormone/insulin-like growth factor axis, Hypothalamic-pituitary-thyroid axis, Tris (2-butoxyethyl) phosphate, Zebrafish
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Down-Regulation
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Flame Retardants/toxicity*
- Growth Hormone/genetics
- Heart Rate/drug effects
- Hypothalamo-Hypophyseal System/drug effects
- Hypothalamo-Hypophyseal System/embryology
- Larva/drug effects
- Organophosphorus Compounds/toxicity*
- Thyroid Gland/drug effects
- Thyroid Gland/embryology
- Thyroxine/genetics
- Triiodothyronine/genetics
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 28772162 Full text @ Aquat. Toxicol.
- CTD
- 28772162
Citation
Liu, Y., Wu, D., Xu, Q., Yu, L., Liu, C., Wang, J. (2017) Acute exposure to tris (2-butoxyethyl) phosphate (TBOEP) affects growth and development of embryo-larval zebrafish. Aquatic toxicology (Amsterdam, Netherlands). 191:17-24.
Abstract
Tris (2-butoxyethyl) phosphate (TBOEP), is used as a flame retardant worldwide. It is an additive in materials and can be easily discharged into the surrounding environment. There is evidence linking TBOEP exposure to abnormal development and growth in zebrafish embryos/larvae. Here, using zebrafish embryo as a model, we investigated toxicological effects on developing zebrafish (Danio rerio) caused by TBOEP at concentrations of 0, 20, 200, 1000, 2000μg/L starting from 2h post-fertilization (hpf). Our findings revealed that TBOEP exposure caused developmental toxicity, such as malformation, growth delay and decreased heart rate in zebrafish larvae. Correlation analysis indicated that inhibition of growth was possibly due to down-regulation of expression of genes related to the growth hormone/insulin-like growth factor (GH/IGF) axis. Furthermore, exposure to TBOEP significantly increased thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in whole larvae. In addition, changed expression of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis was observed, indicating that perturbation of HPT axis might be responsible for the developmental damage and growth delay induced by TBOEP. The present study provides a new set of evidence that exposure of embryo-larval zebrafish to TBOEP can cause perturbation of GH/IGF axis and HPT axis, which could result in developmental impairment and growth inhibition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping