PUBLICATION
MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish
- Authors
- Zhang, J., Liu, W., Jin, Y., Jia, P., Jia, K., Yi, M.
- ID
- ZDB-PUB-170803-6
- Date
- 2017
- Source
- Scientific Reports 7: 7055 (Journal)
- Registered Authors
- Yi, Meisheng
- Keywords
- Development, Zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Germ Cells/metabolism*
- Gonads/embryology*
- In Situ Hybridization
- MicroRNAs/metabolism*
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- PubMed
- 28765643 Full text @ Sci. Rep.
Citation
Zhang, J., Liu, W., Jin, Y., Jia, P., Jia, K., Yi, M. (2017) MiR-202-5p is a novel germ plasm-specific microRNA in zebrafish. Scientific Reports. 7:7055.
Abstract
Gametogenesis is a complicated biological process by which sperm and egg are produced for genetic transmission between generations. In many animals, the germline is segregated from the somatic lineage in early embryonic development through the specification of primordial germ cells (PGCs), the precursors of gametes for reproduction and fertility. In some species, such as fruit fly and zebrafish, PGCs are determined by the maternally provided germ plasm which contains various RNAs and proteins. Here, we identified a germ plasm/PGC-specific microRNA miR-202-5p for the first time in zebrafish. MiR-202-5p was specifically expressed in gonad. In female, it was expressed and accumulated in oocytes during oogenesis. Quantitative reverse transcription PCR and whole mount in situ hybridization results indicated that miR-202-5p exhibited a typical germ plasm /PGC-specific expression pattern throughout embryogenesis, which was consistent with that of the PGC marker vasa, indicating that miR-202-5p was a component of germ plasm and a potential PGC marker in zebrafish. Our present study might be served as a foundation for further investigating the regulative roles of miRNAs in germ plasm formation and PGC development in zebrafish and other teleost.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping