PUBLICATION

Dynamics of actinotrichia regeneration in the adult zebrafish fin

Authors

König, D., Page, L., Chassot, B., Jaźwińska, A.

ID

ZDB-PUB-170802-15

Date

2017

Source

Developmental Biology 433(2): 416-432 (Journal)

Registered Authors

Keywords

actinodin, blastema, extracellular matrix, lepidotrichia, osteoblasts, wound epithelium

MeSH Terms

Osteoblasts/metabolism
Animals
Extracellular Matrix/metabolism
Regeneration/physiology*
Animal Structures/metabolism
Animal Structures/ultrastructure
Mesoderm
Homeostasis
Wound Healing/physiology
Animal Fins/physiology*
Animal Fins/ultrastructure
Gene Expression Regulation
Zebrafish Proteins/biosynthesis
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Zebrafish/genetics
Zebrafish/physiology*
Collagen/metabolism
Collagen/ultrastructure

(all 19)

PubMed

28760345 Full text @ Dev. Biol.

Abstract

The skeleton of adult zebrafish fins comprises lepidotrichia, which are dermal bones of the rays, and actinotrichia, which are non-mineralized spicules at the distal margin of the appendage. Little is known about the regenerative dynamics of the actinotrichia-specific structural proteins called Actinodins. Here, we used immunofluorescence analysis to determine the contribution of two paralogous Actinodin proteins, And1/2, in regenerating fins. Both proteins were detected in the secretory organelles in the mesenchymal cells of the blastema, but only And1 was detected in the epithelial cells of the wound epithelium. The analysis of whole mount fins throughout the entire regenerative process and longitudinal sections revealed that And1-positive fibers are complementary to the lepidotrichia. The analysis of another longfin fish, a gain-of-function mutation in the potassium channel kcnk5b, revealed that the long-fin phenotype is associated with an extended size of actinotrichia during homeostasis and regeneration. Finally, we investigated the role of several signaling pathways in actinotrichia formation and maintenance. This revealed that the pulse-inhibition of either TGFβ/Activin-βA or FGF are sufficient to impair deposition of Actinodin during regeneration. Thus, the dynamic turnover of Actinodin during fin regeneration is regulated by multiple factors, including the osteoblasts, growth rate in a potassium channel mutant, and instructive signaling networks between the epithelium and the blastema of the regenerating fin.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB	amputation: caudal fin, chemical treatment: EC 2.7.10.1 (receptor protein-tyrosine kinase) inhibitor	Adult	fin regeneration decreased efficacy, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: EC 2.7.10.1 (receptor protein-tyrosine kinase) inhibitor	Adult	regenerating fin and1 expression decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: EC 2.7.10.1 (receptor protein-tyrosine kinase) inhibitor	Adult	regenerating fin and2 expression decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: EC 2.7.10.1 (receptor protein-tyrosine kinase) inhibitor	Adult	regenerating fin ab2-and1 labeling decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: EC 2.7.10.1 (receptor protein-tyrosine kinase) inhibitor	Adult	regenerating fin decreased length, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: SB 431542	Adult	fin regeneration decreased efficacy, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: SB 431542	Adult	regenerating fin and1 expression decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: SB 431542	Adult	regenerating fin and2 expression decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: SB 431542	Adult	regenerating fin ab2-and1 labeling decreased amount, abnormal	Fig. 7
AB	amputation: caudal fin, chemical treatment: SB 431542	Adult	regenerating fin decreased length, abnormal	Fig. 7

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
ty86d		Point Mutation	kcnk5b
zf132Tg	Tg(Ola.Sp7:NLS-GFP)	Transgenic Insertion
zp3		Point Mutation	fgf20a

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB
fgf20a^zp3/zp3
kcnk5b^ty86d/ty86d
pd1Tg
WT
zf132Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

König et al., 2017 ZDB-PUB-170802-15 PMID:28760345

Dynamics of actinotrichia regeneration in the adult zebrafish fin

König et al., 2017

ZDB-PUB-170802-15
PMID:28760345