PUBLICATION

Heat indicators of oxidative stress, inflammation and metal transport show dependence of cadmium pollution history in the liver of female zebrafish

Authors
Zhu, Q.L., Guo, S.N., Yuan, S.S., Lv, Z.M., Zheng, J.L., Xia, H.
ID
ZDB-PUB-170802-1
Date
2017
Source
Aquatic toxicology (Amsterdam, Netherlands)   191: 1-9 (Journal)
Registered Authors
Keywords
Cadmium pre-exposure, Fish, High temperature, Immunity, Metal transport, Oxidative stress
MeSH Terms
  • Animals
  • Biological Transport
  • Biomarkers/metabolism
  • Cadmium/metabolism
  • Cadmium/toxicity*
  • Dose-Response Relationship, Drug
  • Female
  • Gene Expression/drug effects
  • Gene Expression/immunology
  • Hot Temperature*
  • Inflammation
  • Liver/drug effects*
  • Liver/enzymology
  • Liver/immunology
  • Oxidative Stress/drug effects*
  • Oxidative Stress/genetics
  • Oxidative Stress/immunology
  • Water Pollutants, Chemical/metabolism
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish*/immunology
  • Zebrafish*/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
28763775 Full text @ Aquat. Toxicol.
Abstract
Environmental stressors such as high temperature and metal exposure may occur sequentially, simultaneously, previously in aquatic ecosystems. However, information about whether responses to high temperature depend on Cd exposure history is still unknown in fish. Zebrafish were exposed to 0 (group 1), 2.5 (group 2) and 5μg/L (group 3) cadmium (Cd) for 10 weeks, and then each group was subjected to Cd-free water maintained at 26°C and 32°C for 7days respectively. 26 indicators were used to compare differences between 26°C and 32°C in the liver of female zebrafish, including 5 biochemical indicators (activity of Cu/Zn-SOD, CAT and iNOS; LPO; MT protein), 8 molecular indicators of oxidative stress (mRNA levels of Nrf2, Cu/Zn-SOD, CAT, HSF1, HSF2, HSP70, MTF-1 and MT), 5 molecular indicators of inflammation (mRNA levels of IL-6, IL-1β, TNF-α, iNOS and NF-κB), 8 molecular indicators of metal transport (mRNA levels of, ZnT1, ZnT5, ZIP8, ZIP10, ATP7A, ATP7B and CTR1). All biochemical indicators were unchanged in group 1 and changed in group 2 and 3. Contrarily, differences were observed in almost all of molecular indicators of inflammation and metal transport in group 1, about half in group 2, and few in group 3. We also found that all molecular indicators of oxidative stress in group 2 and fewer in group 1 and 3 were significantly affected by heat. Our data indicated that heat indicators of oxidative stress, inflammation and metal transport showed dependence of previous cadmium exposure in the liver of zebrafish, emphasizing metal pollution history should be carefully considered when evaluating heat stress in fish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping