PUBLICATION

Screening in larval zebrafish reveals tissue-specific distributions of fifteen fluorescent compounds

Authors
Yao, Y., Sun, S., Fei, F., Wang, J., Wang, Y., Zhang, R., Wu, J., Liu, L., Liu, X., Cui, Z., Li, Q., Yu, M., Dang, Y., Wang, X.
ID
ZDB-PUB-170730-3
Date
2017
Source
Disease models & mechanisms   10(9): 1155-1164 (Journal)
Registered Authors
Li, Qiang, Wang, Jingjing, Wang, Xu, Wang, Youhua
Keywords
Bone staining, Drug screening, Epirubicin, Hyperplasia, Purpurin, Zebrafish
MeSH Terms
  • Animals
  • Anthraquinones/metabolism
  • Bone Density/drug effects
  • Bone and Bones/drug effects
  • Bone and Bones/metabolism
  • Doxorubicin/pharmacology
  • Epirubicin/metabolism
  • Fluorescent Dyes/metabolism*
  • Hyperplasia
  • Larva/drug effects
  • Larva/metabolism
  • Mice, Inbred C57BL
  • Oncogenes
  • Organ Size/drug effects
  • Organ Specificity/drug effects
  • Tissue Distribution/drug effects
  • Zebrafish/metabolism*
PubMed
28754836 Full text @ Dis. Model. Mech.
Abstract
The zebrafish is a prominent vertebrate model for low-cost in vivo whole organism screening. In our recent screening of the distribution patterns of fluorescent compounds in live zebrafish larvae, fifteen compounds with tissue-specific distributions were identified. Several compounds were observed to accumulate in tissues where they were reported to induce side-effects, and compounds with similar structures tended to be enriched in the same tissues, with minor differences. In particular, we found three novel red fluorescent bone-staining dyes: purpurin, lucidin and 3-hydroxy-morindone; purpurin can effectively label bones in both larval and adult zebrafish, as well as in postnatal mice, without significantly affecting bone mass and density. Moreover, two structurally similar chemotherapeutic compounds, doxorubicin and epirubicin, were observed to have distinct distribution preferences in zebrafish. Epirubicin maintained a relatively higher concentration in the liver, and performed better in inhibiting hepatic hyperplasia caused by the over-expression of krasG12V In total, our study suggests that the transparent zebrafish larvae serve as valuable tools for identifying tissue-specific distributions of fluorescent compounds.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes