PUBLICATION
The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos
- Authors
- Ko, E.Y., Cho, S.H., Kwon, S.H., Eom, C.Y., Jeong, M.S., Lee, W., Kim, S.Y., Heo, S.J., Ahn, G., Lee, K.P., Jeon, Y.J., Kim, K.N.
- ID
- ZDB-PUB-170727-8
- Date
- 2017
- Source
- Fish & shellfish immunology 68: 525-529 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Inflammation, NF-κB, Pro-inflammatory mediator, ROS, Zebrafish embryos
- MeSH Terms
-
- Acetylcysteine/metabolism
- Animals
- Embryo, Nonmammalian
- Fish Diseases/immunology*
- Fish Proteins/metabolism*
- Gene Expression Regulation
- Inflammation/immunology
- Inflammation/veterinary*
- Inflammation Mediators/metabolism*
- Lipopolysaccharides/administration & dosage
- NF-kappa B/metabolism*
- Pyrrolidines/metabolism
- Reactive Oxygen Species/metabolism*
- Thiocarbamates/metabolism
- Zebrafish*
- PubMed
- 28743626 Full text @ Fish Shellfish Immunol.
Citation
Ko, E.Y., Cho, S.H., Kwon, S.H., Eom, C.Y., Jeong, M.S., Lee, W., Kim, S.Y., Heo, S.J., Ahn, G., Lee, K.P., Jeon, Y.J., Kim, K.N. (2017) The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos. Fish & shellfish immunology. 68:525-529.
Abstract
In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. IκB was suppressed by PDTC, but not significantly. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping