PUBLICATION
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
- Authors
- Zhang, Y., Qin, W., Lu, X., Xu, J., Huang, H., Bai, H., Li, S., Lin, S.
- ID
- ZDB-PUB-170726-11
- Date
- 2017
- Source
- Nature communications 8: 118 (Journal)
- Registered Authors
- Huang, Haigen, Lin, Shuo
- Keywords
- CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
- MeSH Terms
-
- Genetic Engineering/methods
- Fetal Proteins/genetics
- Zebrafish Proteins/genetics
- CRISPR-Cas Systems/genetics*
- Base Sequence
- Gene Editing/methods*
- Sequence Homology, Amino Acid
- Point Mutation
- Zebrafish/genetics*
- Mutagenesis, Site-Directed/methods*
- Growth Differentiation Factor 6/genetics
- Amino Acid Sequence
- Reproducibility of Results
- T-Box Domain Proteins/genetics
- Sequence Homology, Nucleic Acid
- Animals
- PubMed
- 28740134 Full text @ Nat. Commun.
Citation
Zhang, Y., Qin, W., Lu, X., Xu, J., Huang, H., Bai, H., Li, S., Lin, S. (2017) Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nature communications. 8:118.
Abstract
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping