PUBLICATION

Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish

Authors
Yin, G., Cao, L., Du, J., Jia, R., Kitazawa, T., Kubota, A., Teraoka, H.
ID
ZDB-PUB-170720-12
Date
2017
Source
The Journal of Toxicological Sciences   42: 455-459 (Journal)
Registered Authors
Teraoka, Hiroki
Keywords
Glucocorticoid, Hepatomegaly, Liver, Steatosis, Zebrafish
MeSH Terms
  • Animals
  • Dexamethasone/toxicity*
  • Disease Models, Animal*
  • Fatty Liver/chemically induced*
  • Fatty Liver/drug therapy
  • Fatty Liver/genetics
  • Gene Knockdown Techniques
  • Glucocorticoids/toxicity*
  • Hepatomegaly/chemically induced*
  • Hepatomegaly/drug therapy
  • Hepatomegaly/genetics
  • Mifepristone/pharmacology
  • Mifepristone/therapeutic use
  • Receptors, Glucocorticoid/antagonists & inhibitors
  • Receptors, Glucocorticoid/genetics
  • Receptors, Glucocorticoid/physiology
  • Time Factors
  • Zebrafish*
PubMed
28717104 Full text @ J. Toxicol. Sci.
CTD
28717104
Abstract
Fish hepatobiliary syndrome, characterized by hepatomegaly and fatty liver, has been frequently reported in many cultured fish species and has caused a dramatic economic loss in China. Glucocorticoids are thought to be important non-nutritional factors for hepatomegaly and fatty liver development. In the present study, a dexamethasone-induced zebrafish model of fatty liver and hepatomegaly was established, and the role of glucocorticoid receptor (GR) in the development of hepatomegaly and fatty liver was investigated using developing zebrafish. Exposure of larval zebrafish at 5 days post fertilization (dpf) to dexamethasone for 24 hr caused significant increases of liver size and number of fish with hepatic steatosis at 6 dpf. The increase of liver size caused by dexamethasone was significantly reversed by treatment with RU486, a GR antagonist, and by gene knock-down with a morpholino against the GR. The dexamethasone-induced hepatic steatosis was also inhibited by treatment with RU486. Overall, the results highlight larval zebrafish as a useful model for stress-induced liver failure.
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