PUBLICATION
Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish
- Authors
- Yin, G., Cao, L., Du, J., Jia, R., Kitazawa, T., Kubota, A., Teraoka, H.
- ID
- ZDB-PUB-170720-12
- Date
- 2017
- Source
- The Journal of Toxicological Sciences 42: 455-459 (Journal)
- Registered Authors
- Teraoka, Hiroki
- Keywords
- Glucocorticoid, Hepatomegaly, Liver, Steatosis, Zebrafish
- MeSH Terms
-
- Animals
- Dexamethasone/toxicity*
- Disease Models, Animal*
- Fatty Liver/chemically induced*
- Fatty Liver/drug therapy
- Fatty Liver/genetics
- Gene Knockdown Techniques
- Glucocorticoids/toxicity*
- Hepatomegaly/chemically induced*
- Hepatomegaly/drug therapy
- Hepatomegaly/genetics
- Mifepristone/pharmacology
- Mifepristone/therapeutic use
- Receptors, Glucocorticoid/antagonists & inhibitors
- Receptors, Glucocorticoid/genetics
- Receptors, Glucocorticoid/physiology
- Time Factors
- Zebrafish*
- PubMed
- 28717104 Full text @ J. Toxicol. Sci.
- CTD
- 28717104
Citation
Yin, G., Cao, L., Du, J., Jia, R., Kitazawa, T., Kubota, A., Teraoka, H. (2017) Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish. The Journal of Toxicological Sciences. 42:455-459.
Abstract
Fish hepatobiliary syndrome, characterized by hepatomegaly and fatty liver, has been frequently reported in many cultured fish species and has caused a dramatic economic loss in China. Glucocorticoids are thought to be important non-nutritional factors for hepatomegaly and fatty liver development. In the present study, a dexamethasone-induced zebrafish model of fatty liver and hepatomegaly was established, and the role of glucocorticoid receptor (GR) in the development of hepatomegaly and fatty liver was investigated using developing zebrafish. Exposure of larval zebrafish at 5 days post fertilization (dpf) to dexamethasone for 24 hr caused significant increases of liver size and number of fish with hepatic steatosis at 6 dpf. The increase of liver size caused by dexamethasone was significantly reversed by treatment with RU486, a GR antagonist, and by gene knock-down with a morpholino against the GR. The dexamethasone-induced hepatic steatosis was also inhibited by treatment with RU486. Overall, the results highlight larval zebrafish as a useful model for stress-induced liver failure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping