PUBLICATION

Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools

Authors
Förster, D., Arnold-Ammer, I., Laurell, E., Barker, A.J., Fernandes, A.M., Finger-Baier, K., Filosa, A., Helmbrecht, T.O., Kölsch, Y., Kühn, E., Robles, E., Slanchev, K., Thiele, T.R., Baier, H., Kubo, F.
ID
ZDB-PUB-170714-9
Date
2017
Source
Scientific Reports   7: 5230 (Journal)
Registered Authors
Arnold-Ammer, Irene, Baier, Herwig, Barker, Alison, Filosa, Alessandro, Finger-Baier, Karin, Helmbrecht, Thomas, Kölsch, Yvonne, Kubo, Fumi, Kühn, Enrico, Laurell, Eva, Robles, Estuardo, Slanchev, Krasimir, Thiele, Tod
Keywords
Genetic markers, Neural circuits, Retina
MeSH Terms
  • Brain/physiology*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Animals
  • Genes, Reporter
  • Gene Expression Regulation, Developmental
  • Gene Targeting*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/growth & development
  • Animals, Genetically Modified/metabolism
  • Transgenes*
  • Chromosomes, Artificial, Bacterial*
  • Neurons/physiology*
(all 17)
PubMed
28701772 Full text @ Sci. Rep.
Abstract
Genetic access to small, reproducible sets of neurons is key to an understanding of the functional wiring of the brain. Here we report the generation of a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes and neuropeptides, were selected according to their expression patterns in small and unique subsets of neurons from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used to generate driver lines that label neuronal populations in patterns that, to a large but variable extent, recapitulate the endogenous gene expression. We used image registration to characterize, compare, and digitally superimpose the labeling patterns from our newly generated transgenic lines. This analysis revealed highly restricted and mutually exclusive tissue distributions, with striking resolution of layered brain regions such as the tectum or the rhombencephalon. We further show that a combination of Gal4 and Cre transgenes allows intersectional expression of a fluorescent reporter in regions where the expression of the two drivers overlaps. Taken together, our study offers new tools for functional studies of specific neural circuits in zebrafish.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mpn101TgTransgenic Insertion
    mpn128TgTransgenic Insertion
      mpn155TgTransgenic Insertion
        mpn200TgTransgenic Insertion
          mpn201TgTransgenic Insertion
            mpn202TgTransgenic Insertion
              mpn203TgTransgenic Insertion
                mpn204TgTransgenic Insertion
                  mpn205TgTransgenic Insertion
                    mpn206TgTransgenic Insertion
                      1 - 10 of 29
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      CreEFGCre
                      DendraEFGDendra
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GCaMPEFGGCaMP
                      mCherryEFGmCherry
                      TagRFPEFGTagRFP
                      TomatoEFGTomato
                      1 - 9 of 9
                      Show
                      Mapping
                      No data available
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                      Citation
                      Förster, D., Arnold-Ammer, I., Laurell, E., Barker, A.J., Fernandes, A.M., Finger-Baier, K., Filosa, A., Helmbrecht, T.O., Kölsch, Y., Kühn, E., Robles, E., Slanchev, K., Thiele, T.R., Baier, H., Kubo, F. (2017) Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools. Scientific Reports. 7:5230.