PUBLICATION
Resveratrol ameliorates diet-induced dysregulation of lipid metabolism in zebrafish (Danio rerio)
- Authors
- Ran, G., Ying, L., Li, L., Yan, Q., Yi, W., Ying, C., Wu, H., Ye, X.
- ID
- ZDB-PUB-170708-6
- Date
- 2017
- Source
- PLoS One 12: e0180865 (Journal)
- Registered Authors
- Keywords
- Zebrafish, Autophagic cell death, Lipid metabolism, Body weight, Blood plasma, Blood sugar, Muscle proteins, Obesity
- MeSH Terms
-
- Female
- Caloric Restriction/adverse effects
- PPAR gamma/genetics
- PPAR gamma/metabolism
- Eating/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Caveolin 1/genetics
- Caveolin 1/metabolism
- Antioxidants/pharmacology*
- Microtubule-Associated Proteins/genetics
- Microtubule-Associated Proteins/metabolism
- Signal Transduction
- Zebrafish
- Diet/methods*
- Lipid Metabolism/drug effects*
- Lipid Metabolism/genetics
- Phosphorylation
- Sirtuin 1/genetics
- Sirtuin 1/metabolism
- Protein Kinases/genetics
- Protein Kinases/metabolism
- Gene Expression Regulation/drug effects*
- Stilbenes/pharmacology*
- Animals
- Triglycerides/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Male
- PubMed
- 28686680 Full text @ PLoS One
Citation
Ran, G., Ying, L., Li, L., Yan, Q., Yi, W., Ying, C., Wu, H., Ye, X. (2017) Resveratrol ameliorates diet-induced dysregulation of lipid metabolism in zebrafish (Danio rerio). PLoS One. 12:e0180865.
Abstract
Defective lipid metabolism is associated with increased risk of various chronic diseases, such as obesity, cardiovascular diseases, and diabetes. Resveratrol (RSV), a natural polyphenol, has been shown the potential of ameliorating disregulations of lipid metabolism. The objective of this study was to investigate the effects of feed intake and RSV on lipid metabolism in zebrafish (Danio rerio). The adult males were randomly allocated to 6 groups: control (Con, 8 mg cysts/fish/day), control with 20 ?mol/L RSV (Con+RSV), calorie restriction (CR, 5 mg cysts/fish/day), calorie restriction with RSV (CR+RSV), overfeed (OF, 60 mg cysts/fish/day), and overfeed with RSV (OF+RSV) groups. The treatment period was 8 weeks. Results showed that CR reduced body length, body weight, and condition factor of zebrafish. CR reduced levels of plasma triglyceride (TG) and induced protein expression of phosphorylated AMP-activated protein kinase-? (pAMPK?), silent information regulator 2 homolog 1 (Sirt1), and peroxisome proliferator activated receptor gamma coactivator-1? (PGC1?). RSV attenuated CR-induced pAMPK?/AMPK?increases. RSV increased levels of Sirt1 protein in the OF zebrafish, and decreased OF-induced increase in peroxisome proliferator-activated receptor-? (PPAR?) protein level. Additionally, RSV down-regulated caveolin-1 and up-regulated microtubule-associated protein 1 light chain 3 -II (LC3-II) protein levels in OF zebrafish. In conclusion, these results suggest that 1) CR reduces plasma TG level through activation of the AMPK?-Sirt1- PGC1? pathway; 2) under different dietary stress conditions RSV might regulate AMPK phosphorylation bi-directionally; 3) RSV might regulate lipid metabolism through the AMPK?-Sirt1-PPAR? pathway in OF zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping