PUBLICATION
The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.
- Authors
- Vinberg, F., Wang, T., De Maria, A., Zhao, H., Bassnett, S., Chen, J., Kefalov, V.J.
- ID
- ZDB-PUB-170627-5
- Date
- 2017
- Source
- eLIFE 6: (Journal)
- Registered Authors
- Keywords
- mouse, neuroscience, rhesus macaque, zebrafish
- MeSH Terms
-
- Animals
- Antiporters/deficiency
- Antiporters/metabolism*
- Mice, Inbred C57BL
- Mice, Knockout
- Primates
- Retinal Cone Photoreceptor Cells/physiology*
- Sodium-Calcium Exchanger/metabolism*
- Vision, Ocular*
- Zebrafish
- PubMed
- 28650316 Full text @ Elife
Citation
Vinberg, F., Wang, T., De Maria, A., Zhao, H., Bassnett, S., Chen, J., Kefalov, V.J. (2017) The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.. eLIFE. 6.
Abstract
Calcium (Ca2+) plays an important role in the function and health of neurons. In vertebrate cone photoreceptors, Ca2+ controls photoresponse sensitivity, kinetics, and light adaptation. Despite the critical role of Ca2+ in supporting the function and survival of cones, the mechanism for its extrusion from cone outer segments is not well understood. Here, we show that the Na+/Ca2+, K+ exchanger NCKX4 is expressed in zebrafish, mouse, and primate cones. Functional analysis of NCKX4-deficient mouse cones revealed that this exchanger is essential for the wide operating range and high temporal resolution of cone-mediated vision. We show that NCKX4 shapes the cone photoresponse together with the cone-specific NCKX2: NCKX4 acts early to limit response amplitude, while NCKX2 acts late to further accelerate response recovery. The regulation of Ca2+ by NCKX4 in cones is a novel mechanism that supports their ability to function as daytime photoreceptors and promotes their survival.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping